摘要
目的研究趋化因子受体4(CCR4)在肾透明细胞癌(ccRCC)组织中的表达与临床预后关系,及对ccRCC细胞株786-0增殖、侵袭和迁移能力的影响。方法选择2013年3月—2018年9月新疆医科大学第一附属医院病理诊断为ccRCC患者(63例)的肾脏组织标本为研究组,14例正常肾脏组织标本为对照组,采用免疫组织化学方法检测两组CCR4的表达,分析CCR4阳性表达与阴性表达患者预后生存(OS)。利用Western blot技术检测不同ccRCC细胞株CCR4表达情况,CCR4干扰慢病毒沉默CCR4基因的表达后,将ccRCC细胞株786-0分为3组:786-0、786-0/siRNA、786-0/control细胞,利用MTT法检测3组细胞增殖能力,细胞划痕实验和Transwell侵袭实验检测细胞迁移和侵袭能力,并进行比较分析。结果研究组CCR4阳性表达率为52.38%(33/63),对照组CCR4阳性表达率为21.43%(3/14),差异有统计学意义(χ^2=4.408,P=0.036)。Kaplan-Meier生存曲线分析显示CCR4是ccRCC患者OS的影响因素(χ^2=9.319,P=0.002)。ccRCC细胞株786-0沉默CCR4基因后,对ccRCC细胞增殖无影响,但使细胞的迁移侵袭能力减弱。结论CCR4在ccRCC组织中呈现高表达,且与ccRCC患者临床预后密切相关,其可能通过影响肿瘤细胞的侵袭转移能力,进而影响肿瘤的发生与进展,CCR4可能成为ccRCC的潜在治疗靶点。
Objective To investigate the relationship between the expression of chemokine receptor 4(CCR4)in renal clear cell carcinoma(ccRCC)and clinical prognosis,and its effect on the proliferation,invasion and migration of human renal cell line 786-0.Methods The renal tissue specimens of 63 patients pathologically diagnosed as ccRCC from March 2013 to September 2018 in the First Affiliated Hospital of Xinjiang Medical University were selected as the study group and 14 normal renal tissue specimens as the control group.The expression of CCR4 in the two groups was detected by immunohistochemistry,and the prognosis survival(OS)of CCR4 positive expression and negative expression was analyzed.Western blot was used to detect the expression of CCR4 in different ccRCC cell lines.After interfering with the expression of lentiviral silencing CCR4 gene,the renal cell line 786-0 was divided into three groups:786-0,786-0/siRNA and 786-0/control cells.The proliferation ability of the three groups was detected by MTT method.The cell scratch test and the Transwell invasion test were used to detect the cell migration and invasion ability,and carried on comparative analysis.Results The positive CCR4 expression rate was 33/63(52.38%)in the study group and 3/14(21.43%)in the control group,the difference was statistically significant(χ^2=4.408,P=0.036).Kaplan-Meier survival curve analysis showed that CCR4 was the influencing factor of OS in the patients with ccRCC(χ^2=9.319,P=0.002).After the CCR4 gene was silenced by RCC cell line 786-0,the proliferation of RCC cells was not affected,but the migration and invasion ability of RCC cells were weakened.Conclusion CCR4 is highly expressed in ccRCC tissues and is closely related to the clinical prognosis of ccRCC patients.It may affect the occurrence and progression of tumors by affecting the invasion and metastasis ability of tumor cells,and may become a potential therapeutic target for ccRCC.
作者
李晓东
王文光
闫燊燊
刘强
王玉杰
LI Xiaodong;WANG Wenguang;YAN Shenshen;LIU Qiang;WANG Yujie(Department of Urology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
出处
《新疆医科大学学报》
CAS
2020年第12期1525-1530,共6页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81060210)
新疆维吾尔自治区自然科学基金(2017D01C294)。
关键词
肾透明细胞癌
侵袭性
CCR4
siRNA干扰技术
Renal carcinoma cell(RCC)
invasive
chemokine receptor 4(CCR4)
siRNA interference technique
