摘要
目的确定毛樱桃炎痛贴的制备工艺,并对其抗炎、镇痛活性进行评价.方法提取、分离长白山毛樱桃中的抗炎活性组分,测定炎痛贴的初黏力,通过均匀性、剥离强度、皮肤黏力、涂布性等指标优选贴剂的基质处方,制备毛樱桃炎痛贴.剪取毛樱桃炎痛贴外敷于小鼠背部脱毛区,1次/d,连续4 d,最后1次给药30 min后,将小鼠耳部涂抹巴豆油致炎,观察耳肿胀度,通过热板试验计算痛阈提高百分率.结果毛樱桃炎痛贴的最优处方:毛樱桃提取物0.11 g,丙三醇3.0 g,聚丙烯酸钠NP-7000.5 g,卡波姆9400.2 g,水10 mL,氮酮0.2 g;毛樱桃炎痛贴对不同给药组小鼠巴豆油致耳肿胀抑制率分别是32.10%、39.48%、49.72%;毛樱桃炎痛贴在给药1 h时镇痛作用最佳,低中高剂量组对热板致痛小鼠痛阈提高百分率分别是21.50%、31.34%、39.99%.结论本试验确定了毛樱桃炎痛贴的最佳制备工艺,且毛樱桃炎痛贴能有效抑制小鼠巴豆油耳肿胀,具有较强的镇痛作用.
Objective To determine the preparation technology of Prunus tomentosa thumb Yantong Plaster,and evaluate its anti-inflammatory and analgesic activities.Method Extract and isolate the anti-inflammatory components from Changbaishan Prunus tomentosa thumb.Determinate the initial adhesive force of Yantong Plaster.The matrix prescription of the plaster was optimized by uniformity,peeling strength,skin adhesion and coating to prepare Prunus tomentosa thumb Yantong Plaster.The plaster were applied to the depilated area of the back of mice,once a day for four days.The ear of mice was smeared with croton oil to induce inflammation for the last administration after 30 min,and the degree of ear swelling was observed and the percentage of pain threshold increase was calculated.Results The optimal prescription of Prunus tomentosa thumb Yantong Plaster is that Prunus tomentosa thumb extraction 0.11 g,glycerin 3.0 g,sodium polyacrylate NP-7000.5 g,Carbomer 9400.2 g,water 10 mL,azone 0.2 g.The inhibition rates of Prunus tomentosa thumb Yantong Plaster on the ear swelling induced by croton oil in mice were 32.10%,39.48%and 49.72%,respectively.The best analgesic effect of Prunus tomentosa thumb Yantong Plaster was after 1 hour administration.The percentage of pain threshold increase of low,middle and high dose group was 21.50%,31.34%and 39.99%respectively.Conclusion This experiment has determined the best preparation technology of Prunus tomentosa thumb Yantong Plaster,and Prunus tomentosa thumb Yantong Plaster can effectively inhibit the swelling of croton oil ear in mice and has a strong analgesic effect.
作者
古虹
范紫微
邹天琪
张铮旭
孙利娟
陈曦
张成义
GU Hong;FAN Ziwei;ZOU Tianqi;ZHANG Zhengxu;SUN Lijuan;CHEN Xi;ZHANG Chengyi(Pharmacy College of Beihua University,Jilin 132013,China;Basic Medical College of Beihua University,Jilin 132013,China)
出处
《北华大学学报（自然科学版）》
CAS
2020年第6期734-739,共6页
Journal of Beihua University（Natural Science）
基金
吉林省科技攻关计划项目(20190304048YY)
吉林省卫生健康委员会基金项目(2019J046)
吉林市科技创新发展计划项目(201820847)
北华大学研究生创新计划项目(2019053,2019064).
关键词
毛樱桃
炎痛贴
配方
制备
抗炎
镇痛
Prunus tomentosa thumb
Yantong Plaster
formula
preparation
anti-inflammation
analgesia
