摘要
肺癌是世界范围内发病率和死亡率较高的恶性肿瘤之一,严重威胁着国民的生命安全与健康。肺癌的早期诊断是肺癌预防和治疗过程中的关键环节,对肺癌进行早期诊断有利于提高患者的生存率。外泌体(exosomes)与肿瘤的侵袭与转移过程密切相关,在肺癌的发生发展过程中,外泌体发挥着重要的调控作用。近年来,以外泌体为载体的生物标记物成为肺癌强有力的诊断工具。外泌体是一种由细胞分泌的由膜包裹的大小均一、直径约为30 nm-200 nm的脂质双分子层结构小囊泡。外泌体的内容物包含不同类型的核酸和蛋白质,这些核酸和蛋白质来源于其亲本细胞(包括亲本癌细胞),具有广泛的生理功能,包括参与免疫调节、细胞间联络等。外泌体中的生物大分子物质,如单链RNA、长非编码RNA、微小RNA(microRNA,miRNA)、蛋白质以及脂类,可以为肺癌的早期临床诊断提供有价值的信息。因此,本文就外泌体的来源、结构特点、提取方法、生物学特性和在肺癌早期诊断中的作用研究进展做简要阐述。
Lung cancer is a malignant tumor with high incidence and mortality in the world,which seriously threatens people’s safety and health.Early diagnosis of lung cancer is the key part in the process of prevention and treatment of lung cancer.It can improve the survival of patients with lung cancer.Exosomes are closely related to the invasion and metastasis process of tumor,it plays an important role in the development of lung cancer.Biomarkers based on exosomes have become a powerful diagnostic tool of lung cancer.Exosomes are lipid bilayer vesicles with uniform size and diameter of 30 nm-200 nm secreted by cells.Exosomes contain different types of nucleic acids and proteins.These nucleic acids and proteins are derived from their parent cells(including parent cancer cells),which have a wide range of physiological functions,including immune regulation,intercellular communication and other physiological activities.Biomacromolecules in exosomes,such as singlestranded RNA,long noncoding RNA,microRNA,protein and lipids,which can provide valuable genetic information for early clinical diagnosis of lung cancer.This review described the origin,structural characteristics,extraction methods,biological characteristics of exosomes and the relationships of exosomes in the early diagnosis of lung cancer.
作者
罗丹
李春雷
武伦
陈琴华
Dan LUO;Chunlei LI;Lun WU;Qinhua CHEN(Department of Medical Experimental Center,Affiliated Dongfeng Hospital,Hubei University of Medicine,Shiyan 442008,China;Hubei University of Medicine,College of Pharmacy,Shiyan 442000,China;Shenzhen Baoan Authentic Traditional Chinese Medicine Therapy Hospital,Shenzhen S18102,China)
出处
《中国肺癌杂志》
CAS
CSCD
北大核心
2020年第11期999-1006,共8页
Chinese Journal of Lung Cancer
基金
国家自然科学基金(No.81872509)
湖北医药学院研究生科技创新项目(No.YC2019021)资助。
关键词
外泌体
肺肿瘤
早期诊断
Exosomes
Lung neoplasms
Early diagnosis
