摘要
目的:研究褐腐天麻与土壤微生物菌群的组成及变化,阐述天麻种植过程中天麻褐腐病与土壤微生物菌群之间的关系,为揭示天麻褐腐病的原因提供理论基础。方法:采用内转录间隔区(ITS)和16S rDNA高通量测序技术,分别对健康、褐腐天麻及其周际土壤中真菌和细菌的微生物多样性、群落组成结构、群落结构相似性进行分析。结果:与健康组相比,褐腐天麻及其土壤中真菌和细菌分类单元(OTUs)数目均有增加,且土壤中真菌、细菌丰富度和多样性显著增高,褐腐天麻真菌多样性显著降低,细菌多样性显著增高。在属分类水平上,健康天麻及其周际土壤中的优势真菌属为被孢霉属Mortierella,该属在褐腐天麻及其土壤中分别减少7.62%和15.75%,优势细菌属为慢生根瘤菌属Bradyrhizobium和Burkholderia-Paraburkholderia;褐腐天麻及其周际土壤中的优势真菌属为土赤壳属Ilyonectria,优势细菌属分别为沙雷氏菌属Serratia和慢生根瘤菌属Bradyrhizobium。结论:褐腐天麻与其周际土壤中真菌菌群具有极高相似度,说明土壤微生物真菌群落变化在一定程度上造成天麻褐腐病的发生,其中褐腐天麻真菌群落组成中致病真菌土赤壳属Ilyonectria成为优势菌属,推测该菌属可能含有天麻褐腐病的致病菌。
Objective:To study the microbial community composition and diversity of brown-rot Gastrodia elata and its surface soil,in order to explain the relationship between brown-rot G.elata and soil microflora in G.elata planting process and provide theoretical basis for revealing the reasons of G.elata brownrot disease.Method:Used internal transcribed spacer region(ITS)and 16S rDNA high-throughput sequencing technologies to detect the microbial diversity,community structure composition and community structure similarity of fungi and bacteria in healthy tuber,Brown-rot tuber,healthy soil and Brown-rot soil.Result:Compared with health groups,the number of fungi and bacteria operational taxonomic units(OTUs)was increased in brown-rot G.elata and its soil,and the abundance and diversity of fungi and bacteria in brown-rot G.elata soil were significantly decreased.The diversity of fungi in the tubers of brown-rot G.elata was significantly reduced,while the diversity of bacteria was significantly decreased.At the genus level,Mortierella was dominant fungi genus in healthy tuber and healthy soil,which was reduced 7.62%and 15.75%respectively in brown-rot tuber and brown-rot soil.And the dominant bacteria genus was Bradyrhizobium and BurkholderiaParaburkholderia respectively.Ilyonectria was dominant fungi genus in brown-rot tuber and brown-rot soil,the dominant bacteria genus was Serratia and Bradyrhizobium respectively.Conclusion:The fungal flora in the tuber of brown-rot G.elata had a very high degree of similarity to that in the surrounding soil.These results indicated that the change of soil microbial fungal community caused the occurrence of G.elata brown-rot disease to a certain extent.And the pathogenic fungal Ilyonectria was dominant genus in fungi community of brown-rot tuber and brown-rot soil.Ilyonectria may have the main G.elata brown-rot disease pathogen.
作者
唐鑫
张进强
滕力庆
周涛
袁青松
江维克
郭兰萍
徐娇
王晓
张光文
潘成
TANG Xin;ZHANG Jin-qiang;TENG Li-qing;ZHOU Tao;YUAN Qing-song;JIANG Wei-ke;GUO Lan-ping;XU Jiao;WANG Xiao;ZHANG Guang-wen;PAN Cheng(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Resource Center for Chinese Meteria Medica,Chinese Academy of Chinese Medical Sciences,Beijing 100700,China;Shandong Analysis and Test Center,Ji'nan 250014,China;Guizhou Wumeng Teng Fungus Industry Limited Company,Dafang 551600,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第24期153-160,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81960694)
中央本级重大增减支项目(2060302)
贵州省科技计划项目(黔科合平台人才[2019]5611号)
贵州省高层次创新型百层次人才项目(黔科合平台人才[2018]5638)
贵州省教育厅创新群体重大研究项目(黔教合KY字[2018]022)
现代农业产业技术体系项目(CARS-21)。
关键词
天麻
土壤
微生物多样性
褐腐病
Gastrodia elata
soil
microbial diversity
brown-rot
