摘要
目的探讨诱导痰半乳糖凝集素-3结合蛋白(Galectin-3BP)及相关炎症因子水平检测在中性粒细胞型哮喘诊断中的价值。方法选择2018年10月~2019年6月江汉大学附属医院呼吸与危重症医学科收治的65例哮喘患者为研究对象,其中中性粒细胞型哮喘组(NA)27例,嗜酸粒细胞型哮喘组(EA)38例,ELISA法检测两组患者诱导痰Galectin-3BP的表达水平,并分析Galectin-3BP与诱导痰炎症细胞及炎症因子的相关性。结果NA组诱导痰Galectin-3BP水平与EA组比较(102.86±24.08ng/ml vs 64.02±18.93ng/ml),差异有统计学意义(t=47.314,P<0.05)。直线相关分析显示诱导痰Galectin-3BP与IL-1β,IL-6和IL-8均呈正相关(r=0.670,0.632和0.651,均P<0.001),与诱导痰中性粒细胞、巨噬细胞及淋巴细胞数均呈正相关(r=0.475,0.461和0.454,均P<0.05),与嗜酸性粒细胞数无明显相关性(r=-0.219,P>0.05)。结论诱导痰Galectin-3BP在NA患者中高表达,且与中性粒细胞相关炎症因子呈正相关,可作为NA的生物标志物。
Objective To investigatethe value of induced sputum galectin-3BP and related inflammatory factors in the diagnosis of neutrophil asthma.Methods 65 cases of patients with asthma from Respiratory Medicine of Wuhan No.6 Hospital Affiliated to Jianghan University from October 2018 to June 2019 were selected,including 27 neutrophilic cases and 36 eosinophilic cases.The concentrations of Galectin-3BP and inflammatory cytokines in sputum were measured by ELISA.The correlations among Galectin-3BP and airway inflammation were analyzed.Results Sputum Galectin-3BP was increased in NA compared with EA(102.86±24.08ng/ml vs 64.02±18.93ng/ml),the differences were statistically significant(t=47.314,P<0.05).Galectin-3BP was significantly positively associated with IL-1β,IL-6 and IL-8(r=0.670,0.632,0.651,all P<0.001),and positively associated with neutrophils,macrophages and lymphocytes(r=0.475,0.461,0.454,all P<0.05),while there was no significant correlation with eosinophil number(r=-0.219,P>0.05).Conclusion Induced sputum Galectin-3BP was highly expressed in NA patients,which ispositively correlatedto neutrophil-associated inflammatory factors and can be used as a biomarker for NA.
作者
刘敏
李承红
刘双
王小江
钟金男
石奕
LIU Min;LI Cheng-hong;LIU Shuang;WANG Xiao-jiang;ZHONG Jin-nan;SHI Yi(Department of Respiratory Medicine,Wuhan No.6 Hospital,Affiliated Hospital of Jianghan University,Wuhan 430015,China)
出处
《现代检验医学杂志》
CAS
2020年第6期56-58,128,共4页
Journal of Modern Laboratory Medicine
