摘要
【目的】建立高丹草组织培养再生体系,为高丹草转基因育种研究奠定基础。【方法】试验以高丹草成熟种子为外植体,采用MS基本培养基,研究不同外源激素配比(2,4-D、NAA、6-BA、IBA)对愈伤组织、不定芽诱导分化和组培苗生根的影响。【结果】适宜的愈伤组织诱导培养基为MS+2,4-D(1.5 mg/L),诱导率达到65.63%;芽分化培养基为MS+NAA(0.5 mg/L)+6-BA(1.5 mg/L),分化率为80.21%;生根培养基为MS+IBA(1.0 mg/L),生根率达74.30%;移栽组培苗选择花土∶珍珠岩∶蛭石=3∶1∶1的营养土作为移栽基质,生长状况较好。【结论】高丹草成熟种子的再生体系为诱导培养基MS+2,4-D(1.5 mg/L),芽分化培养基为MS+NAA(0.5 mg/L)+6-BA(1.5 mg/L),生根培养基为MS+IBA(1.0 mg/L)。
【Objective】To establish a tissue culture and regeneration system of Sorghum-Sudangrass for the research of Sorghum-Sudangrass transgenic breeding.【Methods】The experiment used MS as the basic medium,and the mature seeds of Sorghum-Sudangrass were used as explants to study the effect of different exogenous hormone ratios(2,4-D,NAA,6-BA,IBA)on callus,induction and differentiation of adventitious buds and influence of seedling rooting.【Results】The suitable callus induction medium was MS+2,4-D(1.5 mg/L),and the induction rate reached 65.63%;adventitious bud inductions MS medium with NAA(0.5 mg/L)and 6-BA(1.5 mg/L)was optimal,with a adventitious bud induction rate of 80.21%;the optimal rooting medium was MS medium containing 1.0 mg/L IBA,with a rooting rate of 74.30%;for transplanting tissue culture seedlings,the nutrient soil of flower soil∶perlite∶vermiculite=3∶1∶1 was selected as the transplanting substrated.【Conclusion】The regeneration system of mature seeds of Sorghum-Sudangrass was induction medium MS+2,4-D(1.5 mg/L),and the bud differentiation medium was MS+NAA(0.5 mg/L)+6-BA(1.5 mg/L),and rooting medium was MS+IBA(1.0 mg/L).
作者
石悦
房永雨
于卓
杨东升
柴文娟
刘拴成
SHI Yue;FANG Yongyu;YU Zhuo;YANG Dongsheng;CHAI Wenjuan;LIU Shuancheng(College of Life Science,Jining Normal University,Jining 012000,China;College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019,China;Institute of Grassland,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)
出处
《北方农业学报》
2020年第5期119-124,共6页
Journal of Northern Agriculture
基金
集宁师范学院博士科研启动基金项目(jsbsjj1901)
国家自然科学基金(31772644)
内蒙古自治区自然科学基金项目(2018BS03011)。
关键词
高丹草
成熟种子
组织培养
愈伤组织
Sorghum-Sudangrass
Mature seeds
Tissue culture
Callus
