摘要
以种子为外植体,MS为基本培养基,添加不同浓度的6-BA、NAA和IBA进行罗布麻种子愈伤组织诱导及植株的再生研究。结果表明,愈伤组织诱导的最适培养基为MS+6-BA(2 mg/L)+NAA(0.4 mg/L);幼苗分化培养基为:MS+6-BA(2 mg/L)+NAA(0.2 mg/L);最适生根培养基为:MS+IBA(0.5 mg/L)。这一组培体系的建立,为内蒙古地区的野生罗布麻的快繁利用奠定了基础。
With Apocynum seeds as explant and MS as basic medium,callus induction and plant regeneration were studied by adding different concentrations of 6-BA,NAA and IBA.The results showed that the optimal medium of callus induction was：MS＋6-BA（2 mg/L）＋NAA（0.4 mg/L）;the medium of plantlets differentiation was：MS＋6-BA（2 mg/L）＋NAA（0.2 mg/L）;the optimal medium of rooting was：MS＋IBA（0.5 mg/L）.The system of tissue culture laid a foundation for reasonable exploitation and utilization of wild Apocynum venetum Linn.in Inner Mongolia.
出处
《种子》
CSCD
北大核心
2010年第4期5-8,共4页
Seed
基金
内蒙古自然科学基金项目(2007110200508)资助
关键词
罗布麻
种子
愈伤组织诱导
植株再生
Apocynum
seeds
callus tissue induction
plant regeneration
