摘要
本试验旨在研究饲料中添加壳寡糖(COS)对克氏原螯虾(Procambarus clarkii)消化酶活性、肠道菌群结构、血清非特异性免疫指标及抗病力的影响。选用240尾初始体重为(17.39±1.20)g的克氏原螯虾,随机分为4组,每组3个重复,每个重复20尾。各组分别饲喂添加0(C0组)、200(C200组)、400(C400组)和800 mg/kg(C800组)壳寡糖的试验饲料。试验期14 d。结果表明:1)C200组和C800组的肠道淀粉酶活性显著高于C0组(P<0.05),C200组、C400组和C800组的肠道脂肪酶、胰蛋白酶活性及肝胰腺淀粉酶、脂肪酶活性均显著高于C0组(P<0.05),C200组和C400组的肝胰腺胰蛋白酶活性显著高于C0组(P<0.05)。2)C200组的Simpson指数、Chao1指数、ACE指数及Shannon指数均显著高于C0组(P<0.05)。与C0组相比,C200组、C400组和C800组的肠道中变形菌门(Proteobacteria)的相对丰度均有所下降,厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和软壁菌门(Tenericutes)的相对丰度均有所上升;各组肠道中相对丰度较高且共有的菌属有红细菌属(Rhodobacter)、乳球菌属(Lactococcus)、希瓦氏菌属(Shewanella)和拟杆菌属(Bacteroides)。3)C400组和C800组的血清碱性磷酸酶(AKP)活性显著高于C0组(P<0.05),C400组的血清酸性磷酸酶(ACP)活性显著高于C0组(P<0.05),C200组、C400组和C800组的血清过氧化氢酶(CAT)活性均显著低于C0组(P<0.05)。4)白斑综合征病毒(WSSV)攻毒第7天,克氏原螯虾的累积死亡率随着饲料中壳寡糖添加水平的增加而下降。其中,C400组和C800组的累积死亡率相较于C0组分别降低了42.10%和57.89%。由此可见,在饲料中添加适量的壳寡糖可以提高克氏原螯虾消化酶活性,改善肠道菌群结构,并提高抗WSSV感染的能力。
This study was conducted to investigate the effects of dietary chitosan oligosaccharide(COS)on digestive enzyme activities,intestinal microflora structure,serum non-specific immune indices and disease resistance of Procambarus clarkii.A total of 240 Procambarus clarkii with an initial body weight of(17.39±1.20)g were selected and randomly divided into 4 groups with 3 replicates per group and 20 crayfish per replicate.Crayfish in the 4 groups were feed diets supplemented with 0(C0 group),200(C200 group),400(C400 group)and 800 mg/kg(C800 group)chitosan oligosaccharide,respectively.The experiment lasted for 14 days.The results showed as follows:1)the intestinal amylase activity of C200 group and C800 group was significantly higher than that of C0 group(P<0.05),the intestinal lipase and trypsin activities and hepatopancreas amylase and lipase activities of C200 group,C400 group and C800 group were significantly higher than those of C0 group(P<0.05),and the hepatopancreas trypsin activity of C200 group and C400 group was significantly higher than that of C0 group(P<0.05).2)The Simpson index,Chao1 index,ACE index and Shannon index of C200 group were significantly higher than those of C0 group(P<0.05).Compared with C0 group,the relative abundance of Proteobacteria of C200 group,C400 group and C800 group was decreased,while the relative abundances of Bacteroidetes,Firmicutes and Tenericutes were increased;the Rhodobacter,Lactococcus,Shewanella and Bacteroides had higher relative abundances and common bacterial genus in all the 4 groups.3)The serum alkaline phosphatase(AKP)activity of C400 group and C800 group was significantly higher than that of C0 group(P<0.05),the serum acid phosphatase(ACP)activity of C400 group was significantly higher than that of C0 group(P<0.05),and the serum catalase(CAT)activity of C200 group,C400 group and C800 group was significantly lower than that of C0 group(P<0.05).4)At day 7 of white spot syndrome virus(WSSV)challenge,the cumulative mortality rate of Procambarus clarkii was decreased wi
作者
张诗雨
谢梦琪
许荔立
吴志新
田丁
袁军法
陈孝煊
ZHANG Shiyu;XIE Mengqi;XU Lili;WU Zhixin;TIAN Ding;YUAN Junfa;CHEN Xiaoxuan(College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention,Wuhan 430070,China;National Demonstration Center for Experimental Aquaculture Education,Huazhong Agricultural University,Wuhan 430070,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2020年第12期5864-5874,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
湖北省技术创新专项(2018ABA103)
重点研发计划(2019YFC1606002)。
关键词
壳寡糖
克氏原螯虾
消化酶
肠道菌群
抗病力
chitosan oligosaccharide
Procambarus clarkii
digestive enzyme
intestinal microflora
disease resistance
