摘要
为研究沙门菌感染对小鼠白细胞总数及其亚群动态变化的影响,采取腹腔注射的方法给小鼠接种6.5×10^9 CFU/mL浓度的沙门菌0.1 mL/只,分别在4、6、8、12、16和24 h采集小鼠血液,应用全自动动物血液细胞分析仪检测感染小鼠的白细胞总数、淋巴细胞数、单核细胞数和粒细胞数,记录并分析小鼠24 h内白细胞总数及其亚群的动态变化。结果表明:模型组小鼠白细胞总数及其亚群均呈先升高后降低的趋势,且白细胞总数和粒细胞数的变化幅度最明显,与空白对照组比较差异极显著(P<0.01)。说明感染沙门菌能够刺激小鼠机体产生免疫应答,影响血液中白细胞总数及其亚群的动态变化。
The present study aimed to assess the effect of Salmonella infection on the dynamic changes of the total number of white blood cells and their subgroups in mice.This study adopted intraperitoneal injection to inoculate mice with Salmonella at a concentration of 6.5×10^9 CFU/mL.The blood samples of mice were collected at 4,6,8,12,16 and 24 h,respectively,and detected by an automatic animal blood cell analyzer.The total number of white blood cells,the number of lymphocytes,the number of monocytes,and the number of granulocytes in the infected mice were recorded and analyzed for the dynamic changes of the total number of white blood cells and their subgroups within 24 h.The results showed that the total number of white blood cells and their subgroups all increased in the initial stage of infection and then decreased,and the total number of white blood cells and the number of granulocytes had the most significant changes,which were significantly(P<0.01)different from the blank control group.Mice Salmonella infection can stimulate the immune response of mice and affect the dynamic changes of the total number of white blood cells and their subgroups in the blood.
作者
孙善闻
赵彤
谢艺萌
蒋鹏轩
王新
SUN Shan-wen;ZHAO Tong;XIE Yi-meng;JIANG Peng-xuan;WANG Xin(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing163319,China)
出处
《畜牧与饲料科学》
2020年第6期13-16,31,共5页
Animal Husbandry and Feed Science
关键词
沙门菌
小鼠
白细胞总数
粒细胞
淋巴细胞
单核细胞
Salmonella
mice
total number of white blood cells
granulocytes
lymphocytes
monocytes
