摘要
目的建立一种简便、稳定、符合临床特征的小鼠感染大肠杆菌致细菌性腹膜炎的模型。方法小鼠腹腔分别注射9种浓度的2株大肠杆菌菌液,分别于4、6、8、16、24h记录小鼠外周血白细胞总数的变化;记录实验组小鼠死亡时间,并解剖死亡小鼠,观察其腹腔情况以及肝、脾组织的病理变化。结果各实验组小鼠均表现出细菌性腹膜炎临床征象,高于2×108cfu/ml浓度的实验组小鼠细菌性腹膜炎尤为典型;小鼠外周血白细胞总数随着注射菌液后时间的延长呈现先升高后降低的趋势;病理检查显示实验组小鼠肝、门管、脾窦内有不同程度的中性粒细胞浸润。标准菌株的MLD为3×108cfu/ml。结论小鼠大肠杆菌细菌性腹膜炎模型建立成功;小鼠死亡率因不同菌株、不同浓度而有所差异。
Objective To establish a simple, stable animal model of bacterial peritonitis,which was similar to clinical syndrome. Methods Mice were given intraperitoneal injection of two different strains of Escherichia coli. Body temperature, leukocyte count of mice were closely observed at 4.6,8.16.24h, respectively. The time of death was recorded, and the autopsy was performed immediately. Livers and spleens were obtained for pathological examination. Results The experiment groups showed the typical clinical syndrome of peritonitis, especially those groups beyond 2 × 10^8 cfu/ml; the leukocyte count occupied the tendency from heightening to lower after mice were injected with bacteria. Moreover,the pathological examination of liver,portal canal,sinus lienis revealed inflammatory cells infiltration. The MLD of standard E. coli strain was 3 × 10^8 cfu/ml. Conclusions The acute peritonitis model of Escherichia coli was constructed successfully; The mortality of mice is different in same concentration between different strains.
出处
《齐齐哈尔医学院学报》
2008年第17期2049-2051,共3页
Journal of Qiqihar Medical University
关键词
大肠杆菌
腹膜炎
小鼠
Eseheriehia eoli Peritonitis Mouse
