摘要
探究五大连池碧泊样品中趋磁细菌(Magnetotactic Bacteria,MTB)多样性对不同磁场强度及不同曝磁时间的响应。采用微宇宙培养及高通量测序手段,分别考察不同磁场强度和不同曝磁时间对碧泊样品中MTB Alpha多样性、Beta多样性、群落组成和功能的影响。结果显示,10 Gs磁场下MTB的菌群丰度最高,而50 Gs磁场下MTB的菌群多样性最低;在曝磁第6周MTB的菌群丰度和多样性均最高。结合菌群丰度变化推测长时间曝露在磁场下是引起MTB菌群异化的重要因素。
The diversity of magnetotactic bacteria(MTB)in sediment sample from Bibo respond to different magnetic intensity and processing time were investigated.Microcosmic culture and high-throughput sequencing were used to investigate the effects of different magnetic field intensity and processing time on MTB Alpha diversity,Beta diversity,community composition and community function in Bibo samples.The results showed that the bacteria abundance of MTB in 10 Gs magnetic field was the highest,while that in 50 Gs was the lowest.In the 6 th week of treatment,the abundance and diversity of MTBs were the highest.Combined with the variation of bacterial abundance,it was speculated that long-term exposure under magnetic field was an important factor causing the alienation of MTB bacterial population.
作者
邢维佳
赵丹
张国静
贺双俊
晏磊
Xing Weijia;Zhao Dan;Zhang Guojing;He Shuangjun;Yan Lei(College of Life Science and Technology,Heilongjiang Bayi Agricultural Unverisity,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2020年第6期70-76,共7页
journal of heilongjiang bayi agricultural university
基金
国家自然科学基金面上项目(41471201)
黑龙江农垦总局重点科研项目(HKKY190406)
黑龙江省自然科学基金面上项目(C201442)
黑龙江省青年创新人才培养计划(UNPYSCT-2015086)
国家重点研发计划子课题(2018YFD0800906-03)。
关键词
碧泊露头泉
趋磁细菌
高通量测序
磁场强度
Bibo outcrop springs
magnetotactic bacteria
high-throughput sequencing
magnetic field intensityy
