摘要
目的鉴定人感染猪种布鲁菌,掌握其生物及分子特征,为布鲁菌病的防控提供依据。方法自江苏省首例人感染猪种布鲁菌患者体内分离布鲁菌菌株,采用血清凝集试验进行血清分型;采用普通PCR方法进行布鲁菌特异性基因bcsp-31检测;采用AMOS-PCR方法进行分型序列IS-711鉴定;采用多重PCR方法进行种及生物型鉴定,对wboA基因扩增产物进行测序,并构建系统发育树;采用多位点序列分析(multilocus sequence analysis,MLSA)进行分子分型分析,并与参考菌株进行聚类分析。结果通过血清凝集试验及PCR方法,确认该菌为猪种布鲁菌生物3型。wboA基因测序后,参考序列聚类分析发现与生物3型猪种布鲁菌str.686(CP007719)最为接近。MLSA分型为ST17型(1-6-4-1-5-3-5-2-4)。结论江苏省首次发现人感染猪种布鲁菌生物3型,MLSA分型为ST17型。今后在该地区进行人间布鲁菌病防控的同时,还应积极与畜牧兽医部门合作,加强生猪的检疫、免疫等防治措施。
Objective To identify human infection with Brucella suis,analyze its biological and molecular characteristics,and to provide basis for prevention and control of brucellosis.Methods Brucella suis strains were isolated from the body of the first case of human Brucella suis infection in Jiangsu Province.Serum agglutination test was used for serotyping.The specific gene bcsp-31 of Brucella was detected by PCR.AMOS-PCR was used to identify IS-711.The species and biotypes were identified by multiplex PCR.The wboA gene products were sequenced and phylogenetic tree was constructed.Multilocus sequence analysis(MLSA)was used for molecular typing,and cluster analysis was performed with reference strains.Results The strain was confirmed to be Brucella suis biotype 3 by serum agglutination test and PCR.After sequencing the wboA gene,cluster analysis of the reference sequence showed that the wboA gene was closest to the biotype 3 strain Brucella suis str.686(CP007719).MLSA was typed into ST17(1-6-4-1-5-3-5-2-4).Conclusions Brucella suis biotype 3 is reported in Jiangsu Province for the first time.The MLSA type is ST17.In the future,the prevention and control of human brucellosis should be carried out.We should actively cooperate with the animal husbandry and veterinary department to increase the quarantine,immunization and other control measures.
作者
谈忠鸣
汪秀斌
周伟忠
董晨
周璐
洪捷
钱慧敏
胡建利
鲍倡俊
Tan Zhongming;Wang Xiubin;Zhou Weizhong;Dong Chen;Zhou Lu;Hong Jie;Qian Huimin;Hu Jianli;Bao Changjun(Key Laboratory of Enteric Pathogenic Microbiology,National Health Commission,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;Hangzhou Xiaoshan Yinong Town Community Health Service Center,Hangzhou 311200,China)
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2020年第11期781-785,共5页
Chinese Journal of Endemiology
基金
江苏省医学重点学科(实验室)(ZDXKA2016008)
江苏省疾病预防控制中心“科教强业”工程青年人才项目(JKRC2016005)。
