摘要
【目的】了解昆明市某猪场戊型肝炎病毒感染情况,进一步探究病毒基因型及分子进化情况,为戊型肝炎防控提供参考。【方法】从该猪场随机采集39份新鲜粪便,用逆转录巢式聚合酶链反应对HEV ORF2部分基因片段扩增、克隆及测序;应用生物信息学软件进行同源性分析并构建系统进化树。【结果】PCR检测结果发现:该猪场核酸阳性率达35.9%(14/39)。同源性分析结果表明:14株流行毒株核苷酸序列与GenBank中HEV Ⅰ~Ⅳ型参考株的同源性分别为77.4%~83.9%、81.0%~83.2%、78.8%~86.9%和90.5%~98.5%,全部流行株均属基因Ⅳ型。进化分析结果表明:YN-KM-9与该猪场其他流行毒株同源性较远,应属不同基因亚型。【结论】该猪群HEV Ⅳ感染率较高,对公共卫生安全构成潜在威胁。
[Purpose]To determine the hepatitis E infection situation in a pig farm of Kunming City, Yunnan Province, further explore the virus genotype and molecular evolution, and provide a reference for the prevention and control of hepatitis E.[Method]Thirty-nine fresh fecal samples were randomly collected from the target a pig farm.The reverse transcription nested polymerase chain reaction(RT-nPCR) was used to detect the ORF2 gene of swine hepatitis E virus.After cloning and sequencing, bioinformatics software was used for homology analysis and the phylogenetic tree was constructed.[Result]RT-nPCR detection results found that the positive rate was 35.9%(14/39).The analysis results showed that homologies between 14 detected strains and genotype Ⅰ-Ⅳ represents were 77.4%-83.9%, 81.0%-83.2%, 78.8%-86.9% and 90.5%-98.5%, respectively, indicating that all the prevalence strains belongs to HEV type Ⅳ.Evolutionary analysis showed that YN-KM-9 is away from other prevalence strains and should belong to different sub-genotypes.[Conclusion] The HEV Ⅳ infection rate of this pig herd is relatively high, posing a potential threat to public health safety.
作者
王源
郑晓林
严红亚
段新慧
张以芳
杨莎莎
李文贵
WANG Yuan;ZHENG Xiaolin;YAN Hongya;DUAN Xinhui;ZHANG Yifang;YANG Shasha;LI Wengui(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Yunnan Academy of Animal Science and Veterinary Medicine,Kunming 650224,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2020年第6期1111-1114,共4页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(31160495)。
