摘要
以甜瓜为试验材料,研究了低温胁迫下外源硝普钠(SNP)和蔗糖(SUC)对甜瓜种子萌发、幼苗生长和生理特性的影响,探讨外源SNP和SUC对于缓解甜瓜幼苗低温胁迫伤害的可行性。结果表明,低温胁迫显著影响甜瓜种子萌发、幼苗生长和相关生理指标;适宜浓度的外源SNP和SUC处理均能明显提高低温胁迫下甜瓜种子的萌发、促进幼苗生长,诱导抗氧化酶活性,同时也提高了抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量及抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、脱氢抗坏血酸还原酶(DHAR)和单脱氢抗坏血酸还原酶(MDHAR)等代谢关键酶的活性,降低了过氧化氢(H2O2)和丙二醛(MDA)的积累量及超氧阴离子(O2·-)产生速率。研究表明,外源SNP和SUC处理能增强甜瓜幼苗的耐低温性,且具有剂量效应,通过隶属函数分析比较发现,SNP和SUC最佳浓度分别为200μmol/L和1 mmol/L,且前者施用效果显著优于后者。
In order to explore the feasibility of exogenous sodium nitrate(SNP) and sucrose(SUC) alleviating chilling stress injury of melon seedlings. The melon were used as the material, the SNP and SUC were used carry out on the seed germination, seedling growth and physiological characteristics of under low temperature stress.The results showed that chilling stress significantly affected the seed germination, seedling growth and related indexes of physiological. The suitable concentration of exogenous SNP and SUC could significantly improve the seeds germination of melon, seedling growth and the activities of antioxidant enzymes, besides, the content of As A and GSH and the activities of key metabolic enzymes(APX, GR, DHAR, and MDHAR) were increased, and, the accumulation of hydrogen peroxide(H2 O2) and malondialdehyde(MDA) and the production rate of superoxide anion radica(O2·-) were decreased. The above results indicated that exogenous SNP and SUC treatments can improve the low temperature tolerance of melon and have a dose effect. Through the analysis and comparison of membership functions, the optimum concentrations of SNP and SUC treatment were 200 μmol/L and 1 mmol/L respectively, and the effect of SNP treatment was better than SUC.
作者
刁倩楠
范红伟
张文献
曹燕燕
张永平
Dian Qiannan;Fan Hongwei;Zhang Wenxian;Cao Yanyan;Zhang Yongping(Shanghai Key Laboratory of Proected Horiculural Technology,Horticultural Research Institute,Shanghai Academy of Agriculturnl Sciences,Shanghai,201403;Shanghai Apriculture Technology Extension and Service Center,Shanghai,201103)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第21期7209-7216,共8页
Molecular Plant Breeding
基金
上海市瓜果产业技术体系(沪农科产字(2019)第1号)
上海市农业科学院卓越团队(农科创2017(B-06))
上海市闵行区项目(2019MHC051)共同资助。
