摘要
目的通过痛风颗粒各有效部位群对完全弗氏佐剂诱导的关节炎大鼠抗炎镇痛作用差异,探讨痛风颗粒及其各有效部位群可能的抗炎镇痛作用机制。方法将健康SD大鼠56只,随机分为空白组、模型组、总黄酮组、总有机酸组、总生物碱组、痛风颗粒组和阳性对照组,除空白组外,其余6组均建立关节炎的病理模型。造模成功15 d后,连续灌胃给药30 d。测定关节炎大鼠的踝关节肿胀、白细胞计数、嗜中性粒细胞百分比、血清白细胞介素6(IL-6)、白细胞介素10(IL-10)以及肿瘤坏死因子-α(TNF-α)水平以及关节组织病理形态学。结果与模型组相比,痛风颗粒各有效部位群、痛风颗粒及阳性对照组的踝关节肿胀率均显著下降(P<0.01)。痛风颗粒各有效部位群、痛风颗粒及阳性对照组能减轻纤维组织增生,与模型组相比,痛风颗粒各有效部位群、痛风颗粒及阳性对照组的血液中白细胞计数(WBC)和中性粒细胞百分比(N%)无显著性差异(P>0.05)。与模型组相比,痛风颗粒各有效部位群、痛风颗粒及阳性对照组能显著降低模型大鼠的血清IL-6、TNF-α水平,升高IL-10水平(P<0.05,P<0.01)。结论该研究明确了痛风颗粒有效部位群的抗炎镇痛作用,为进一步临床用药和制剂开发提供依据。
Objective To investigate the anti-inflammatory and analgesic effects of the active fractions of Tongfeng granules on rats with arthritis induced by complete Freund's adjuvant.Methods 56 SD rats were randomly divided into seven groups,blank group,model group,total flavonoids group,total organic acid group,total alkaloid group,Tongfeng granule group and positive control group.Except for the blank group,the remaining 6 groups established joints pathological model of inflammation.15 days after the successful modeling,intragastric drug administration was continued for 30 days.The swelling of ankle joint,WBC,N%,IL-6,IL-10,TNF-αand the histopathology of joint were measured.Results Comparing with the model group,each effective fraction group of Tongfeng granules,Gout granules and positive control group decreased the ankle joint swelling rate significantly(P<0.01)and reduced fibrous tissue proliferation.There was no significant difference in WBC and N%of neutrophils.They significantly reduce the level of serum IL-6 and TNF-α,and increase the level of IL-10(P<0.05,P<0.01).Conclusion This study clarifies the anti-inflammatory and analgesic effects of active fractions of Tongfeng granules and provides a basis for further clinical medication and preparation development.
作者
徐熠
刘静
黄瑾
崔金刚
吴铁军
XU Yi;LIU Jing;HUANG Jin;CUI Jingang;WU Tiejun(Department.of Pharmacy,Yueyang Hospital of Integrated Traditional and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Clinical Research Institute of Integrative Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《药学实践杂志》
CAS
2020年第6期528-532,共5页
Journal of Pharmaceutical Practice
基金
上海市卫计委科研课题-青年项目(20174Y0194)
岳阳中西医结合医院院级基金(2018YJ06)
上海市“医苑新星”青年医学人才培养资助计划-临床药师项目(沪卫计人事【2019】72号)
上海中医药大学第二批临床师资“优教”项目资助(新锐培训计划)。
关键词
痛风颗粒
有效部位群
抗炎
镇痛
gout granule
active fractions
anti-inflammation
analgesia
