摘要
本试验旨在获得广谱高效的霉菌毒素吸附剂。采用十六烷基三甲基溴化铵(CTAB)对钠基蒙脱石(Na-MMT)进行改性,对CTAB改性蒙脱石(CTAB-MMT)采用扫描电子显微镜(SEM)、X射线多晶衍射(XRD)仪和傅里叶变换红外光谱(FTIR)仪进行表征分析,并通过体外吸附试验评价其对霉菌毒素的吸附效果。结果显示:制备CTAB-MMT的最佳工艺条件为CTAB浓度2.4%,反应温度60℃,反应时间2 h,pH 11。与钙基蒙脱石(Ca-MMT)、Na-MMT相比,CTAB-MMT呈颗粒状,表面粗糙,有孔洞出现;d001值增大,层间距变大;CTAB-MMT的FTIR图谱中在1 462、2 850、2 929 cm-1附近出现了吸收峰;CTAB-MMT对黄曲霉毒素B1、玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇和伏马毒素B1的吸附率极显著高于Ca-MMT(P<0.01),对玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇的吸附率极显著高于Na-MMT(P<0.01)。由此可见,CTAB-MMT对霉菌毒素具有更强、更广泛的吸附能力,是比较理想的霉菌素素吸附剂。
The aim of this experiment was to obtain a broad-spectrum and high-efficiency mycotoxin adsorbent through modification of sodium montmorillonite(Na-MMT)using cetyltrimethylammonium bromide(CTAB). Scanning electron microscope(SEM),X-ray diffraction(XRD)instrument and Fourier transform infrared(FTIR)spectrometer were used to analyze the structure of CTAB modified montmorillonite(CTABMMT),and the absorption effect of the CTAB-MMT to mycotoxins was evaluated in vitro. The results showed that the optimum conditions for preparing CTAB-MMT were CTAB concentration 2.4%,reaction temperature60 ℃,reaction time 2 h and pH 11. Compared with calcium montmorillonite(Ca-MMT)and Na-MMT,the CTAB-MMT was granular,with a rough surface and pores. The d001 value was higher and the interlayer spacing was larger in CTAB-MMT than those of Ca-MMT and Na-MMT. The absorption peaks of CTAB-MMT in the FTIR pattern were appeared around 1 462,2 850 and 2 929 cm-1,respectively. The adsorption rates of aflatoxin B1(AFB1),deoxynivalenol(DON),zearalenone(ZEN)and fumonision B1(FB1)by CTABMMT were significantly higher than those by Ca-MMT(P<0.01),and the adsorption rates of DON and ZEN by CTAB-MMT were significantly higher than those by Na-MMT(P<0.01). It is concluded that CTAB-MMT has stronger and more extensive adsorption capacity for mycotoxins,which is a potential mycotoxin adsorbent.
作者
刁亚南
朱连勤
陈甫
朱风华
DIAO Yanan;ZHU Lianqin;CHEN Fu;ZHU Fenghua(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2020年第11期5388-5396,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家重点研发计划子课题(2018YFD0501403-2.1)
山东省现代农业产业技术体系家禽产业创新团队(SDAIT-11-07)。
关键词
蒙脱石
十六烷基三甲基溴化铵
霉菌毒素
结构表征
体外吸附
montmorillonite
cetyltrimethylammonium bromide
mycotoxins
structural characterization
ad⁃sorption in vitro
