摘要
目的:研究石菖蒲及其活性成分-α-细辛醚改善疲劳运动大鼠学习记忆的作用及其机制。方法:80只SD大鼠随机分为正常对照组(A)、单纯运动组(B)、运动+α-细辛醚低、中、高剂量组(C、D、E)、运动+石菖蒲低、中、高剂量治疗组(F、G、H),每组10只。并在疲劳运动开始前2 h分别以0.10、0.50和1.00 mgα-细辛醚,灌胃C、D、E组,以0.12、1.20和4.80 g.kg^-1.wt^-1石菖蒲提取物,灌胃F、G、H组。实验结束后采用水迷宫实验进行学习记忆检测,采用生化法检测SOD、NOS活性和MDA含量,免疫印迹法检测海马nNOS蛋白表达水平。结果:实验后E和H组大鼠逃避潜伏期、海马脑组织MDA含量低于B、C、D、F和G组;穿越平台次数、海马脑组织SOD和NOS活性、nNOS蛋白表达高于B、C、D、F和G组,P均<0.01。A、E、H组大鼠海马脑组织SOD活性依次为A>E>H组,而MDA含量则相反,P均<0.01;E组大海马脑组织NOS活性和nNOS蛋白表达低于A和H组,P<0.01或P<0.05,但A和H组,差异无显著性,P均>0.05。A、E、H组大鼠逃避潜伏期和穿越平台次数,差异均无显著性,P均>0.05。结论:石菖蒲及α-细辛醚改善疲劳运动大鼠学习记忆的作用基本一致,其机制与纠正海马自由基代谢失衡和上调海马nNOS/NO信号有关。
Objective:To study the effects of acorus tatarinowii Schott and its active ingredient-alpha-asarone on learning and memory,free radical metabolism and nNOS/NO signal in hippocampus of rats with fatigue movement.Methods:Eighty SD male rats were randomly divided into eight groups:control group(A),exercise group(B),exercise+alpha-asarone low,middle and high dose treatment group(C,D,E),exercise+acorus tatarinowii Schott low,middle and high dose treatment group(F,G,H),with ten rats in each group.The rats in group C,D and E were administered with alpha-asarone at the doses of 0.10,0.50 and 1.00 mg.kg^-1.WT^-1 by ig.The rats in group F,G and H were administered with the extracts of Acorus tatarinowii Schott of at the doses of 0.12,1.20 and 4.80 g.kg^-1.WT^-1 by ig.Learning and memory of rats were tested by the method of water maze experiment,and the activities of SOD and NOS,the contents of MDA were detected by the biochemical methods,and the expression levels of nNOS protein in hippocampus of rats were tested by the method of Western blot in at the end of the experiment.Results:The escape latency and MDA content in hippocampus of rats in groups E and H were lower than those in groups B,C,D,F and G and the numbers of Plateau crossing,SOD and NOS activities and the expression levels of nNOS protein in hippocampus of rats were higher than those in groups B,C,D,F and G(P<0.01).The activities of SOD in hippocampus of rats in groups A,E and H were A>E>H,whereas the contents of MDA were opposite(P<0.01);the activities of NOS and the expression levels of nNOS protein in hippocampus of group E were lower than those of groups A and H(P<0.01 or P<0.05),but there was no significant difference between groups A and H(P>0.05).There were no significant difference in escape latency and numbers of crossing platform among groups A,E and H(P>0.05).Conclusion:Acorus tatarinowii Schott and alpha-asarone can significantly improve learning and memory of rats with fatigue movement.The mechanism is related to reclaiming the imbalance of free ra
作者
朱梅菊
毛泽华
郭红英
朱洪竹
丁孝民
ZHU Mei-ju;MAO Ze-Hua;GUO Hong-ying;ZHU Hong-zhu;DING Xiao-min(Institute of physical education,Jinggangshan University,Ji’an 343009;Logistics support department,Jinggangshan University,Ji’an 343009;College of continuing education and training,Jinggangshan University,Ji’an 343009,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2020年第4期306-311,共6页
Chinese Journal of Applied Physiology
基金
国家自然科学基金项目(31660291,31360255,31160217)
江西省自然科学基金资助项目(20192BAB205092,20151BAB205070)
井冈山大学博士启动基金资助项目(JZB11043)。
关键词
石菖蒲
Α-细辛醚
学习记忆
自由基
神经型一氧化氮合酶
疲劳运动
大鼠
acorus tatarinowii Schott
alpha-asarone
learning and memory
free radical metabolism
neuronal nitric oxide synthase
fatigue movement
rat
