摘要
【目的】探明在添加Zn条件下,黄菖蒲对Cd的抗氧化酶系统响应机制。【方法】通过水培试验,设置了Cd质量浓度为0(Cd0)、0.5(Cd0.5)、5(Cd5)mg/L,Zn质量浓度为0(Zn0)、50(Zn50)、100(Zn100)、150(Zn150)mg/L,研究了Cd胁迫对黄菖蒲抗氧化酶系统的影响,以及Cd在黄菖蒲植株中的积累特征。【结果】Zn的添加缓解了Cd对黄菖蒲植株的胁迫。Cd胁迫下,黄菖蒲植物根部SOD较叶片处先被激活。Zn的添加使黄菖蒲根中POD活性提高,但是低质量浓度Zn(50 mg/L和100 mg/L)使黄菖蒲叶片中POD活性降低;不添加Cd的情况下,Zn的添加使根CAT活性显著提高,黄菖蒲对Cd的转运系数均大于1。不同质量浓度Zn的添加提高了黄菖蒲地上和地下部分对Cd的富集系数,0.5 mg/L质量浓度Cd处理下,添加50、150 mg/L质量浓度Zn,黄菖蒲对Cd的富集系数得到了增加,地上部分别增加了46.48%、58.71%,地下部分分别增加了43.85%、42.05%;5 mg/L质量浓度Cd处理下,添加50、150 mg/L质量浓度Zn,黄菖蒲对Cd的富集系数得到了增加,地上部分别增加了16.13%、9.77%,地下部分别增加了23.93%、1.89%。【结论】Cd胁迫下,Zn的添加提高了黄菖蒲抗氧化酶系统的活性,且提高了黄菖蒲对Cd的富集系数。
【Background】Soil contamination by Cd due to anthropogenic activities including application of agrichemicals and mining has become a global environmental concern.In China, the national soil pollution survey bulletin in 2014 showed that the national environment under threat and most pollutions originated from cultivated soils to which soil contamination by heavy metals contributed 7%, despite the cultivated land per capita in China being only 1/3 of the world average.Remediating Cd-contaminated soil is hence more pressing in China than in other countries.【Objective】This paper aims to unveil the mechanisms underlying the change in antioxidant enzymes in Iris pseudacorus under Cd stress in response to Zn concentration and its consequence for Cd accumulation in the plant.【Method】The experiment was conducted in hydroponic culture, where the Cd concentration was spiked to a certain level.We then amended the culture by adding Zn at different concentrations.The changes in antioxidant enzymes and the accumulation of Cd in different tissues in the plant were measured.【Result】①Adding zinc alleviated the stress of Cd on Iris pseudacorus;②The SOD enzyme in the root was activated earlier than that in the leaves when the plant was under Cd stress;③Adding zinc increased the POD enzyme activity in the roots, but reduced the POD enzyme activity in the leaves when Zn concentration was in the range of 50~100 mg/L.④When the Cd concentration was 0.05 mg/L, adding Zn significantly boosted the activity of root CAT enzyme.⑤The transport coefficients of Cd in the yellow calamus were all higher than unity.When the Cd concentration was 0.05 mg/L, adding Zn significantly increased the enrichment coefficient of Cd in both aboveground and underground parts of Iris pseudacorus.【Conclusion】Under Cd stress, adding Zn improved the antioxidant enzymes and the enrichment coefficient of Cd in Iris pseudacorus.
作者
马欢欢
高峰
樊向阳
胡超
崔二苹
刘春成
莫宇
张茜
MA Huanhuan;GAO Feng;FAN Xiangyang;HU Chao;CUI Erping;LIU Cuncheng;MO Yu;ZHANG Qian(Farmland Irrigation Research Institute,Chinese Academy of Agricultural Sciences,Xinxiang 453002,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;Key Laboratory of High-efficient and Safe Utilization of Agriculture Water Resources of CAAS,Xinxiang 453002,China)
出处
《灌溉排水学报》
CSCD
北大核心
2020年第11期104-111,共8页
Journal of Irrigation and Drainage
基金
“十三五”国家重点研发计划项目(2017YFC0403503)。
关键词
黄菖蒲
抗氧化酶系统
镉
锌
转运系数
富集系数
Iris pseudacorus
antioxidant enzyme system
cadmium
zinc
enrichment coefficient
translocation factor
