摘要
目的探讨将丹参注射液联合人脂肪干细胞(hADSC)对自体脂肪颗粒植入裸鼠皮下后成活率的影响。方法实验主体部分于2019年5月至2019年8月在中国人民解放军国际和平医院输血科实验室完成。选取24只8周龄健康雌性裸鼠,体重(23±3)g,分为4组,每组6只。A组为植入脂肪颗粒0.5 ml;B组为植入脂肪颗粒0.5 ml+hADSC;C组为丹参注射液0.5 g/(kg·d)+脂肪颗粒0.5 ml;D组为丹参注射液0.5 g/(kg·d)+脂肪颗粒0.5 ml+hADSC。移植术后15 d时每组取3只裸鼠取材,HE染色和免疫组织化学染色计数微血管数量。术后30 d各组剩余3只裸鼠取材,HE染色并测量各标本体积。结果组织学肉眼观察,术后15 d,标本均可见完整的包膜形成,新生毛细血管在包膜表面,包膜与移植体周围组织的分界明显,活动度好,质地硬度中等,以疏松结缔组织与周围组织相连;术后30 d,移植体的体积较移植初缩小,部分组织中可见脂肪液化坏死。术后15 d脂肪移植体免疫组织化学切片血管密度值各组差异有统计学意义(P<0.05)。术后30 d,脂肪移植体体积值各组之间差异有统计学意义(P<0.05)。结论丹参注射液与hADSC的综合作用,比各自单独运用更能有效地促进脂肪颗粒移植体早期血管系统的重建,更有效地提高脂肪颗粒成活率。
Objective To investigate the effect of Salvia miltiorrhiza injection combined with human adipose derived stem cells(hADSC)on the survival rate of autologous fat granules implanted in nude mice.Methods A total of 24 healthy 8 weeks old female nude mice weighing(23±3)g were randomly divided into four groups(n=6):Group A was given fat granules 0.5 ml;Group B:fat granules 0.5 ml+hADSC;Group C:Salvia miltiorrhiza 0.5 g/(kg·d)+fat granules 0.5 ml,and Group D:Salvia miltiorrhiza injection 0.5 g/(kg·d)+fat granules+enrichment.3 nude mice were randomly selected from each group for 15 days after transplantation and stained with conventional HE.Immunohistochemical staining was performed to count the number of microvessels.On the 30th day after surgery,the remaining 3 nude mice in each group were sacrificed.The specimens were stained with HE and the volume of each specimen was measured.Results Graft appearance was observed by naked eye:15 days after the operation,all the specimens were formed completely.The new capillaries were shaped on the surface of the capsule.The boundaries of the capsule and the surrounding tissue were obvious.The activity was good,the hardness of the texture was medium,and the loose connective tissue was connected to the surrounding tissue.On the 30 day after operation,the volume of the graft was smaller than that at the beginning of transplantation,and fat liquefaction and necrosis were seen in some tissues.Blood vessel density values of immunohistochemical sections of fat transplantation in each group 15 days after surgery were compared pairwisely.The differences between the groups were statistically significant(P<0.05).Lsd-t method was used for pairwise comparison of fat graft volume values of each group 30 days after surgery,and the difference between each group was statistically significant(P<0.05).Conclusions The combined use of Salvia miltiorrhiza injection and hADSCs can effectively promote the reconstruction of the early vascular system of the fat granule transplantation and improve the su
作者
苗晓燕
豆舒乾
苗国英
王更银
张开
杨建民
Miao Xiaoyan;Dou Shuqian;Miao Guoying;Wang Gengyin;Zhang Kai;Yang Jianmin(Department of Plastic Surgery,the Affiliated Hospital of Hebei University of Engineering,Handan 056002,China;College of clinical medicine,Hebei University of engineering,Handan 056000,China;Dermatologist,affiliated hospital of hebei university of engineering,Handan 056002,China;Department of Blood Transfusion,PLA International Peace Hospital,Shijiazhuang 050000,China;Department of Orthopedics,Handan Central Hospital,Handan 056001,China;Department of Plastic Surgery,Tsinghua Changgung Hospital,Beijing 102218,China)
出处
《中华医学美学美容杂志》
2020年第5期428-432,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
邯郸市科技局2019年度市级科技专项计划一般项目(19422083011-9)。
关键词
丹参注射液
动物实验
人脂肪干细胞
脂肪颗粒移植
成活率
Dan Shen Zhu She Ye
Animal experimentation
Human adipose-derived stem cells
Fat granule transplantation
Survival rate
