摘要
目的:提高透明颤菌胞外多糖(extracellular polysaccharides of Vitreoscilla,VEPS)的产量,初步研究VEPS的抗肿瘤活性。方法:采用了单因素实验、Plackett-Burman实验、最陡爬坡路径实验、Box-Behnken实验设计以及噻唑蓝MTT法。结果:确定影响透明颤菌产胞外多糖的培养基的成分及最佳配比,并初步研究VEPS的抗肿瘤活性。优化得到的最佳培养基配方为蛋白胨5 g·L-1、乳糖39 g·L-1、KH2PO40.17 g·L-1、K2HPO40.46 g·L-1、(NH4)2SO40.2 g·L-1、复合盐溶液1 mL·L-1(MgSO419.5 g·L-1,MnSO45 g·L-1,FeSO41.4 g·L-1,CaCl20.05 g·L-1),此时透明颤菌胞外多糖产量为(583.6±6.3)mg·L-1,与初始培养基培养条件下的产量相比提高了364.3%,与预测值误差为1.3%。结论:体外抗肿瘤实验表明VEPS有良好的抗肿瘤活性。
Objective:To increase the yield of extracellular polysaccharides from Vitreoscilla(VEPS)and preliminarily study its antitumor activity.Methods:Single factor experiment,Plackett-Burman test,steepest climbing path test,Box-Behnken test design and MTT were used for this study.Results:The composition and optimum ratio of the culture medium affecting the production of VEPS were determined and the antitumor activity of VEPS was studied.The optimized formula of the culture medium was as follows:peptone 5 g·L-1,lactose 39 g·L-1,potassium dihydrogen phosphate 0.17 g·L-1,dipotassium hydrogen phosphate 0.46 g·L-1,ammonium sulfate 0.2 g·L-1,and compound salt solution(magnesium sulfate 19.5 g·L-1,manganese sulfate 5 g·L-1,ferrous sulfate 1.4 g·L-1,and calcium chloride 0.05 g·L-1)1 mL·L-1.Under this condition,the yield of extracellular polysaccharides was(583.6±6.3)mg·L-1.Compared with the initial medium,the yield increased by 364.3%with the error of predicted value 1.3%.Conclusion:In vitro anti-tumor experiments showed that VEPS had good anti-tumor activity.
作者
耿晓琦
范冰倩
丁钦然
杜鹏
郑宇
宋佳
GENG Xiao-qi;FAN Bing-qian;DING Qin-ran;DU Peng;ZHENG Yu;SONG Jia(Tianjin University of Science and Technology,Collge of Biological Engineering,Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin 300457,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2020年第19期2229-2238,共10页
Chinese Journal of New Drugs
基金
天津市农业科技示范推广项目(201701180)
天津科技大学青年创新基金(2016LG10)
天津科技大学大学生实验室创新基金(1704A301)
天津市高等学校大学生创新创业训练计划项目(201810057079)
天津市食品绿色制造及安全校企协同创新实验室建设项目(17PTSYJC0080)。
关键词
透明颤菌
胞外多糖
培养基优化
响应面设计
抗肿瘤活
Vitreoscilla
extracellular polysaccharide
medium optimization
response surface design
antitumor activity
