摘要
目的探讨CD4+CD25+CD127low/-Treg细胞比例及血清TGF-β1和IL-17水平,与血小板输注无效的相关性及其潜在机制。方法对照(健康体检者)组14例,PTR(platelet transfusion refractoriness,PTR)组14例。流式细胞仪检测CD4+CD25+CD127low/-Treg细胞比例。ELISA检测血清TGF-β1和IL-17水平。结果 PTR组和对照组CD4+CD25+CD127low/-Treg细胞比例分别为(3.55±2.46)%、(2.61±1.25)%,两者无显著性差异。PTR组和对照组血清TGF-β1分别为(1 093.06±784.84)pg/mL、(4 870.49±755.13)pg/mL,两者有显著性差异(P<0.000 1)。PTR组和对照组血清IL-17分别为(5.17±2.07)pg/mL、(6.88±5.66)pg/mL,两者无显著性差异。结论免疫性血小板输注无效患者血清TGF-β1表达降低,可能参与免疫性血小板输注无效的发生。
Objective To investigate the correlation and potential mechanism between the CD4+ CD25+CD127low/-Treg cells and serum TGF-β1、IL-17 levels and platelet transfusion refractoriness. Methods 14 patients were in the control(healthy physical examination) group and 14 patients were in the PTR(platelet transfusion refractoriness, PTR) group. CD4+CD25+CD127low/-Treg cells were detected by flow cytometery. Serum TGF-β1 and IL-17 levels were detected by ELISA. Results The ratios of CD4+CD25+CD127low/-Treg cells in the PTR group and the control group were(3.55±2.46)% and(2.61±1.25)%, respectively. And there was no significant difference between them(P>0.05). The serum TGF-β1 in the PTR group and the control group were(1 093.06±784.84) pg/ml and(4 870.49±755.13) pg/ml, respectively. There was significant difference between them(P<0.000 1). The serum IL-17 in the PTR group and the control group was(5.17±2.07) pg/ml and(6.88±5.66)pg/ml, respectively. There was no significant difference between them. Conclusion Serum TGF-β1 expression in patients with platelet transfusion refractoriness may be decreased, which may be involved in the occurrence of platelet transfusion refractoriness.
作者
张盈
刘志伟
宋铁军
张钧
ZHANG Ying;LIU Zhiwei;SONG Tiejun;ZHANG Jun(Sir Run Run Shaw Hospital of Zhejiang University,School of Medicine,Hangzhou 310016,China;Hangzhou Xiasha Hospital)
出处
《中国输血杂志》
CAS
2020年第9期899-902,共4页
Chinese Journal of Blood Transfusion
基金
浙江省基础公益研究计划项目(LGD19H080002)
浙江省血液安全研究重点实验室开放基金(2018KF01、2020KF002)
浙江省医药卫生科技计划(2017KY419)。
