摘要
目的研发一种基于固相凝集技术的血小板交叉配型试剂盒。方法采用聚乙烯亚胺进行包被制备固相凝集反应微孔板;用IgG抗-D致敏O型红细胞后与抗人免疫球蛋白桥接,构建包被抗人免疫球蛋白的一步法指示细胞系统;评估自制试剂盒的各项检测性能指标。结果0.1%的聚乙烯亚胺包被96孔微孔板对血小板具有较好的吸附作用,IgG抗-D和抗人免疫球蛋白制备的一步法指示细胞系统具有较好的指示效果,试剂盒中的固相反应微孔板可在37℃保存1周、4℃保存12个月,指示细胞系统可在4℃保存60 d,自制试剂盒与进口的MASPAT试剂盒在血小板交叉配型中的阳性一致率98.13%、阴性一致率98.98%、总一致率为98.80%。结论所研制的血小板交叉配型试剂盒具有稳定性好、准确度高等优势,与进口同类试剂盒质量相当,可用于不同临床机构的血小板快速交叉配型。
Objective To develop a newly reagent kit for platelets cross-matching test with solid phase techniques.Methods Polyethyleneimine was coated on the bottom inner surface of 96-well microplate.Group O red blood cells sensitized with IgG anti-D were interconnected with anti-human globulin,thus the one-step indicator cell was made.The detection capability of the reagent kit was evaluated in parallel with MASPAT kit.Results 96-well microplate coated with0.1%polyethyleneimine had optimal adherence effect on platelets.One-step indicator cells had an equal performance to traditional indicator cells.96-well microplate coated with polyethyleneimine could be stored at 37℃for one week,4℃for one year;indicator cells could be stored at 4℃for 60 days.Cross-matching positive concordance rate of household reagent kit was 98.13%as compared with MASPAT kit,negative concordance rate was 98.98%,and total concordance rate was 98.80%.Conclusion The household reagent kit for platelets cross-matching test had good stability and high degree of accuracy.It had the equal detection capability with MASPAT kit and could be used in different clinical laboratory.
作者
罗广平
骆宏
张润青
张文春
姬艳丽
付涌水
LUO Guang ping;LUO Hong;ZHANG Run qing;ZHANG Wen chun;JI Yan li;FU Yong shui(Guangzhou Blood Center,the Key Medical Laboratory of Guangzhou,Guangzhou,Guangdong 510095,China)
出处
《热带医学杂志》
CAS
2019年第11期1340-1342,1347,共4页
Journal of Tropical Medicine
基金
广州市科技计划一般项目(201707010442)
广州市医学重点学科建设项目(血液安全重点实验室).
关键词
血小板
交叉配型
固相凝集法
Platelet
Cross-matching
Solid phase techniques
