摘要
目的观察去脂软肝方对非酒精性脂肪性肝病大鼠小肠中有机阴离子转运多肽(OATP) 2B1 mRNA和蛋白表达的影响。方法 36只SD雄性大鼠,适应性喂养1周,随机分为正常组、模型组、辛伐他汀组、去脂软肝方高、中、低剂量组,共6组,每组6只。收集肝组织进行HE染色观察脂肪变性情况,采用全自动生化仪检测ALT、AST、血糖(GLU)、血清胆固醇(CHOL)水平,RT-PCR法检测大鼠小肠组织中OATP2B1 mRNA表达量,Western Blot检测大鼠小肠组织中OATP2B1蛋白表达水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果各组间大鼠肝指数、GLU、CHOL水平比较,差异均有统计学意义(F值分别为10.814、12.298、5.024,P值均<0.05)。各组间大鼠小肠组织OATP2B1 mRNA和蛋白表达比较,差异均有统计学意义(F值分别为13.384、73.025,P值均<0.05)。与正常组相比,模型组OATP2B1 mRNA和蛋白表达显著升高(P值均<0.05);与模型组相比,辛伐他汀组和去脂软肝方高、中剂量组OATP2B1 mRNA表达均显著降低(P值均<0.05);与辛伐他汀组比较,去脂软肝方中、低剂量组OATP2B1 mRNA表达均显著降低(P值均<0.05);与正常组相比,模型组OATP2B1蛋白表达显著增加(P <0.05);与模型组相比,各用药组OATP2B1蛋白表达均显著降低(P值均<0.001);与辛伐他汀组比较,去脂软肝方高、中、低剂量组OATP2B1蛋白表达均显著降低(P值均<0.05)。结论去脂软肝方可能是通过降低OATP2B1的过表达,减缓非酒精性脂肪性肝病的病变程度。
Objective To investigate the effect of Quzhi Ruangan decoction on the mRNA and protein expression of organic anion transporting polypeptide 2B1(OATP2B1)in the small intestine of rats with nonalcoholic fatty liver disease(NAFLD).Methods After 1 week of adaptive feeding,36 male Sprague-Dawley rats were randomly divided into normal group,model group,simvastatin group,and high-,middle-,and low-dose Quzhi Ruangan decoction groups,with 6 rats in each group.Liver tissue was collected and HE staining was used to observe hepatic steatosis;an automatic biochemical analyzer was used to measure the serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),glucose(GLU),and cholesterol(CHOL);RT-PCR and Western blot were used to measure the mRNA and protein expression of OATP2B1 in the small intestine of rats.A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups.Results There were significant differences in liver index,GLU,and CHOL between groups(F=10.814,12.298,and 5.024,all P<0.05),and there were also significant differences in the mRNA and protein expression of OATP2B1 in the small intestine(F=13.384 and 73.025,both P<0.05).Compared with the normal group,the model group had significant increases in the mRNA and protein expression of OATP2B1(both P<0.05);compared with the model group,the simvastatin group and the high-and middle-dose Quzhi Ruangan groups had a significant reduction in the mRNA expression of OATP2B1(all P<0.05);compared with the simvastatin group,the middle-and low-dose Quzhi Ruangan groups had a significant reduction in the mRNA expression of OATP2B1(both P<0.05).Compared with the normal group,the model group had a significant increase in the protein expression of OATP2B1(P<0.05);compared with the model group,all treatment groups had a significant reduction in the protein expression of OATP2B1(all P<0.001);compared with the simvastatin group,the high-,m
作者
张素妍
夏恩蕊
周青丽
张顺贞
ZHANG Suyan;XIA Enrui;ZHOU Qingli;ZHANG Shunzhen(School of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming 650000,China;School of Basic Medical Sciences,Yunnan University of Chinese Medicine,Kunming 650000,China)
出处
《临床肝胆病杂志》
CAS
北大核心
2020年第11期2484-2488,共5页
Journal of Clinical Hepatology
基金
云南省科技厅中医联合专项[2018FF001(-042)]
“赛诺”研发创新基金(30260504100)。
