摘要
文章研究扁桃果皮熊果酸对酒精肝损伤的保护作用,将小鼠分为正常对照组、模型对照组、联苯双酯阳性对照组(150 mg/kg)、熊果酸低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量组,其中对正常对照组、模型对照组给予等体积蒸馏水,对其余组小鼠给予10 mL/kg扁桃果皮熊果酸连续灌胃30天。从第16天起,除正常对照组外,其他各组每天给药结束4 h后用30%乙醇(10 mL/kg)进行灌胃,建立小鼠酒精性肝损伤模型,测定小鼠肝脏指数、扁桃果皮熊果酸对小鼠血清谷氨酸氨基转移酶(Glutamate aminotransferase,ALT)、天门冬氨酸氨基转移酶(Aspartate aminotransferase,AST),超氧化物歧化酶(Superoxide dismutase,SOD)活力及丙二醛(Malonic dialdehyde,MDA)含量和小鼠肝组织SOD、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)活力、MDA和谷胱甘肽(Glutathione,GSH)含量的影响,并用苏木精-伊红染色法(Hematoxylin-eosin staining,HE)进行肝组织病理学切片分析。结果表明,扁桃果皮熊果酸可以有效降低小鼠肝脏指数,小鼠血清ALT、AST活力及MDA含量,提高小鼠血清SOD活力,也可以使小鼠肝组织MDA含量降低,SOD、GSH-PX活力及GSH含量升高,肝脏组织病理损伤减轻。结论扁桃果皮熊果酸对酒精性肝损伤有保护作用。
Article research the protective effect of ursolic acid from Amygdalus communis L.peel on alcoholic liver injury.The mice were divided into normal control group,model control group,positive control group of diphenyl diester(150 mg/kg) and low(100 mg/kg),medium(200 mg/kg) and high( 400 mg/kg) dose groups of ursolic acid.The normal control group and model control group were given distilled water of equal volume,while the other groups were given 10 mL/kg of Amygdalus communis L.peels′ ursolic acid by continuous gavage for 30 days.Starting from the 16th day,except for the normal control group,other groups need to be gavaged with 30%ethanol(10 mL/kg) after four hours of daily administration to establish a mouse model of alcoholic liver injury.The mice liver indexes,pathomorphological changes of liver and the effects of ursolic acid on Glutamate aminotransferase(ALT),Aspartate aminotransferase(AST),Superoxide dismutase(SOD) activity and Malonic dialdehyde(MDA) content were measured,as well as Glutathione peroxidase(GSH-PX) activity,MDA and Glutathione(GSH) content in mice liver tissue.The results showed that compared with the model control group,ursolic acid could effectively reduce the liver index,the activity of ALT and AST and the content of MDA in mice serum,increase the activity of SOD in mice serum,decrease the content of MDA in mice liver tissue,increase the activity of SOD,GSH-PX and the content of GSH,alleviate the pathological damage of liver tissue.Conclusionly,Amygdalus communis L.peels′ ursolic acid has a protective effect on alcoholic liver injury.
作者
黄雪玲
李进
张俊
HUANG Xue-ling;LI Jin;ZHANG Jun(Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Key Laboratory of Plant Stress Biology in Arid Land,College of Life Science,Xinjiang Normal University,Urumqi,Xinjiang,830017,China;Xijier People's Government of Mulei Kazakh Autonomous County,Changji Hui Autonomous Prefecture,Xinjiang Uygur Autonomous Region,Changji,Xinjiang,831900,China)
出处
《新疆师范大学学报（自然科学版）》
2024年第1期76-82,共7页
Journal of Xinjiang Normal University(Natural Sciences Edition)
基金
新疆师范大学生物学重点学科开放基金项目(17SDKD0203)。
关键词
扁桃
熊果酸
肝损伤
肝保护
氧化应激
Amygdalus communis L.
Ursolic acid
Liver injury
Hepatoprotective
Oxidative stress
