摘要
通过平板涂布分离法从病虾中分离到多株弧菌,从中挑取代表性菌株HY3,结合其弧菌检测琼脂平板上的菌落形态特征,16S rRNA和fur基因序列分析,鉴定该菌株为副溶血弧菌。副溶血弧菌HY3携带有tlh基因(不耐热溶血毒素)和对虾急性肝胰腺坏死致病基因pirAVP、pirBVP。攻毒试验显示,副溶血弧菌HY3具有较强的致病性,凡纳滨对虾感染后第7 d累积死亡率近100%。药敏试验结果显示,副溶血弧菌HY3对链霉素、氨苄西林耐药;抗性基因检测显示,副溶血弧菌HY3携带blaTEM、strA-strB等相关抗生素抗性基因。常用剂量的渔用消毒剂高锰酸钾对副溶血弧菌HY3的杀菌效果较好,10 mg/L高锰酸钾溶液处理2 h,杀菌率可达99.99%。本试验结果表明,台州凡纳滨对虾养殖场出现的急性肝胰腺坏死病主要致病菌为副溶血弧菌,且对链霉素、氨苄西林耐药,后续防治该病需避免使用该类药物,可以采用高锰酸钾溶液消毒处理防治。
A pathogenic bacterium strain HY3 was isolated from Pacific white shrimp Litopenaeus vannamei with acute hepatopancreatic necrosis disease(AHPND)symptom collected in Taizhou fishery farm by streak plate method,and identified as Vibrio parahaemolyticus by the characteristics of colony on TCBS plate and the sequence analysis of 16S rRNA and fur gene,aiming to investigate the pathogen of AHPND of Pacific white shrimp and to propose prevention strategy.The HY3 strain had tlh(thermolabile hemolysin),pirAVP and pirBVP genes which caused shrimp AHPND.The pathogenic challenge test showed that the strain had strong pathogenicity,with cumulative mortality rate of nearly 100%for the shrimp on the seventh day after infection.Drug susceptibility test revealed that the strain was resistant to streptomycin and ampicillin.The resistance gene test indicated that related resistance genes blaTEM,strA and strB were detected in the strain by PCR and DNA sequencing.Potassium permanganate,a fishery disinfectant,showed strong bactericidal activity to the strain HY3,with bactericidal rate of 99.99%at 10 mg/L potassium permanganate for 2 h.V.parahaemolyticus was proposed as the main pathogen for the AHPND of Pacific white shrimp in Taizhou,with resistant to streptomycin and ampicillin which is not used in control AHPND in Taizhou.However,potassium permanganate is recommended to be applied as disinfectant to prevent AHPND in Taizhou.
作者
余达勇
陈碧秀
钟永军
粱秀燕
何昕蔚
夏海洋
鲍佳伟
YU Dayong;CHEN Bixiu;ZHONG Yongjun;LIANG Xiuyan;HE Xinwei;XIA Haiyang;BAO Jiawei(Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, China;School of Life Sciences, Taizhou University, Taizhou 318000, China;Zhejiang Hongye Seafood Co., Ltd., Linhai 317000, China)
出处
《水产科学》
CAS
CSCD
北大核心
2020年第6期844-851,共8页
Fisheries Science
基金
浙江省自然科学基金青年基金资助项目(LQ18D050001)
浙江省大学生科技创新活动计划暨新苗人才计划(2019R436011)
台州市科技计划项目(1701ny06)
2018年台州市211人才工程(第三层次择优).
关键词
急性肝胰腺坏死病
凡纳滨对虾
副溶血弧菌
fur基因
药敏试验
acute hepatopancreatic necrosis disease
Litopenaeus vannamei
Vibrio parahaemolyticus
fur gene
drug susceptibility test
