摘要
为拓展水产源产细菌素乳酸菌的开发利用,本研究从水产动物及其生境中分离出乳酸菌261株,以细菌素敏感菌为指示菌,筛选产细菌素菌株,鉴定产生菌,并分析所产细菌素的稳定性。结果表明:(1)5株菌上清液排除有机酸后仍对指示菌有抑制作用,与对照组(pH 6.0)相比,菌株G7-1发酵上清经排酸处理后,抑菌率仅降低了1.59%(P>0.05)。进一步经过氧化氢酶和胃蛋白酶处理后,抑菌率分别显著降低了5.07%和21.54%(P<0.05),表明G7-1发酵上清液的抑菌物质含细菌素或其类似物。(2)经16S rDNA序列同源性分析,鉴定菌株G7-1为屎肠球菌。进一步检测其细菌素的稳定性发现,G7-1所产细菌素对热不稳定,但对酸碱处理有较好的耐受性。
In order to improve the development and application of bacteriocin-producing Lactic acid bacteria from aquatic sources,the present study isolated 261 bacteriocin-producing Lactic acid bacteria strains from aquatic animals and their habitats.Using a bacteriocin-sensitive bacteria as theindicator bacteria,bacteriocin-producing strains were screened,identified,and the stability of its bacteriocin was analyzed.The results showed that:(1)The supernatant of 5 out of the isolated strains had the inhibitory effect against the bacteriocin-sensitive indicator bacteria after neutralizing the organic acid.Compared with the control group(pH 6.0),the bacteriostatic effect of fermentation supernatant from G7-1 was reduced only 1.59%after neutralization(P>0.05).Further treatment with catalase and pepsin of G7-1 supernatant significantly(P<0.05)reduced the inhibition rate by 5.07%and 21.54%,respectively;(2)According to the 16S rDNA sequence homology analysis,G7-1 was identified as Enterococcus faecium.The results of acid-based tolerance and thermal stability of bacteriocin revealed that the bacteriocin produced by G7-1 was unstable to heat,but had good resistance to acid or alkali treatment.
作者
高欣
刘颖
刘凡湘
王洋
王竞儒
罗云龙
白东清
GAO Xin;LIU Ying;LIU Fanxiang;WANG Yang;WANG Jingru;LUO Yunlong;BAI Dongqing(Aquatic apartment of Tianjin agricultural university,Tianjin 300384,China;Tianjin Key Laboratory of Aquatic ecology and aquaculture,Tianjin 300384,China)
出处
《中国饲料》
北大核心
2020年第21期29-34,共6页
China Feed
基金
国家级大学生创新创业训练计划项目(201910061016)
国家自然基金(青年科学基金项目)“片球菌素PA-1与乳酸协同损伤酸耐受嗜水气单胞菌内外膜的机理”(31702360)
天津市水产产业技术体系创新团队淡水鱼营养岗位专家、加工岗专家的资助。
关键词
抗菌肽
水产养殖
抑菌活性
屎肠球菌
antimicrobial peptide
aquaculture
antibacterial activity
Enterococcus faecalis
