摘要
结合微芯片电泳-电容耦合式非接触电导检测(ME-C4D)与电压控制-场放大样品堆积技术,设计了一种新型的离子在线双富集电泳微芯片。本研究在传统十字电泳微芯片的样品蓄液池后引入第二蓄液池,利用电压控制,实现了离子在线双富集,并以K^+,Na^+,Li^+3种离子为分析物,考察了微芯片的功能。在最优富集与电泳条件下,样品溶液(K^+,Na^+,Li^+)在100 s内成功实现了富集与分离。实验结果表明,K^+,Na^+,Li^+的富集倍数分别为41,37和34倍。3种离子迁移时间的RSD在5.3%~6.2%范围内,峰高的RSD保持在5%以下。
A novel new electrophoresis microchip for ion on-line double concentration was constructed by microchip electrophoresis-capacitively coupled contactless conductive detection(ME-C4 D)and voltage controlfield amplified sample stacking.In this study,an additional reservoir was introduced after the sample reservoir of the traditional cross-electrophoresis microchip,this achieved ion on-line double concentration by using voltage control.The K^+,Na^+,Li^+were utilzied as the analytes to investigate the performance of the microchip.Under the optimal concentration and electrophoresis conditions,the sample solution contained K^+,Na^+,Li^+was successfully enriched and separated within 100 s.The results indicated that the concentration factors of the K^+,Na^+and Li^+were 41,37 and 34 fold.The RSD of the three ions migration time was within 5.3%-6.2%,and the RSD of the peak height was maintained below 5%.
作者
成伟清
陈思嘉
李龙飞
尤晖
CHENG Weiqing;CHEN Sijia;LI Longfei;YOU Hui(School of Electronic Science and Applied Physics,Hefei University of Technology,Hefei 230009;School of Mechanical Engineering,Guangxi University,Nanning 530004)
出处
《分析试验室》
CAS
CSCD
北大核心
2020年第10期1143-1147,共5页
Chinese Journal of Analysis Laboratory
基金
国家科技支撑计划项目(SQ2015BA0800060)
广东省重点领域研发计划项目(2019B020219003)资助。
关键词
微芯片电泳
电容耦合式非接触电导检测
电压控制
离子在线富集
场放大堆积技术
electrophoresis microchip
capacitively coupled contactless conductivity detection
voltage control
ion on-line concentration
field amplified sample stacking
