摘要
目的探讨反复胚胎种植失败与子宫内膜中CD38、CD138阳性表达的相关性。方法(1)收集2017年1月至2017年12月于昆明医科大学第二附属医院生殖科进行单精子卵泡浆内注射-胚胎移植(ICSI-ET)及体外受精-胚胎移植(IVF-ET)助孕的患者夫妇的治疗资料。将患者分为两组,将行胚胎移植术大于等于2个周期均未获得妊娠者设为反复胚胎种植失败组,共纳入患者共72例。将第一周期行IVF-ET/ICSI-ET后获得妊娠的患者设为对照组,共纳入患者共41例。收集两组患者行宫腔镜术的检查结果、子宫内膜病理检测结果,以及子宫内膜组织行CD38、CD138免疫组织化学染色检查的结果;(2)统计分析采用SPSS17.0软件包进行,比较对照组与反复胚胎种植失败组患者宫腔镜检查结果、子宫内膜病理检查结果,以及子宫内膜CD38、CD138的阳性表达率;比较反复种植失败组患者的种植失败次数不同时患者间CD38、CD138的阳性表达率及细胞表达量是否具有差异。结果(1)反复种植失败组与对照组患者宫腔镜检查及常规病理检测中发现异常的概率差异无统计学意义(P> 0.05);(2)反复胚胎种植失败组子宫内膜CD38、CD138的阳性表达率高于对照组(P <0.05);(3)胚胎种植失败3次和2次者子宫内膜CD38、CD138细胞表达量低于种植失败大于3次者(P <0.05)。结论反复胚胎种植失败与子宫内膜中CD38、CD138的阳性表达有关,检测子宫内膜CD38、CD138有助于发现及治疗慢性子宫内膜炎,可对反复胚胎种植失败患者的治疗提供思路和方法。
Objective To investigate the relevance between the repeated implantation failure(RIF)and the expressions endometrial CD38,CD138 using a retrospective analysis. Methods(1)The infertility cases which had accepted In Vitro Fertilization(IVT-ET)/Intracytoplasmic Sperm Injection(ICSI-ET)treatment in the Department of Reproduction of the Second Affiliated Hospital of Kunming Medical University were retrospectively analyzed from the January to the December of 2017. Based on the included and excluded criteria,these cases were put into two groups,and the repeated implantation failure group contained 72 cases. There were 41 cases having successful pregnancy within the first period after IVT-ET treatment. All patients had taken the hysteroscopy examination and endometrium biopsy by using immunohistochemically staining of CD38 and CD138 on endometrium analysis.(2)The clinical material of the subject group of repeated implantation failure and the control group for applying binary logistic analysis on the positive expression of CD38 and CD138, and potential factors relating to repeated implantation failure were studied.(3)Statistical analysis was conducted by using SSPS 17.0 to compare the expression conditions of CD38 and CD138 between the repeated implantation failure group and the control group and to locate the differences on the CD38 and CD138 positive expression rate and the cell expression amount in repeated implantation failure patients. Results 1.There was no statistically significant difference in the probability of abnormal findings in hysteroscopy and routine pathological examination between the repeated implant failure group and the control group(P > 0.05);(2)All positive expression rates of RIF group on CD38,CD138, and/or the above were higher than those of the control group(P < 0.05);(3)The cell expression amounts of CD38 and CD138 in the cases of more than three times implantation failure were higher than those in the twice failure case(P < 0.05).Conclusion(1) There exists a correlation between the endometrial
作者
万瑞雪
陈静思
别嘉
苏娅
孟昱时
WAN Rui-xue;CHEN Jing-si;BIE Jia;SU Ya;MENG Yu-shi(Reproductive Center,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Reproductive Center,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)
出处
《昆明医科大学学报》
CAS
2020年第10期108-113,共6页
Journal of Kunming Medical University
基金
昆明医科大学“十三五”校级学科建设重点项目(J1301819)
云南省科技厅昆明医科大学应用基础研究联合专项基金资助项目(2017FE467)。
