摘要
目的分析鞍山市2017—2019年肾综合征出血热宿主动物病毒的携带情况,以及重点人群汉坦病毒IgG抗体的检出情况,为制定防控措施提供依据。方法参照《全国肾综合征出血热监测方案(试行)》中的检测方法,用免疫荧光法检测鼠肺抗原,用酶联免疫吸附实验(ELISA法)检测鼠血总抗体和重点人群汉坦病毒IgG抗体。结果免疫荧光法测鼠带毒率,2017—2019年鼠带毒率分别为9.5%、13%、3%,呈现村外大于村内的生境特点,春季高于秋季的季节性特点;ELISA法测定鼠感染率,2017—2019年鼠感染率分别为16.5%、10%、6%,呈现村内大于村外的生境特点,秋季高于春季的季节性特点;ELISA法测定重点人群IgG抗体阳性率,2017—2019年分别为7.23%、0、12.2%。结论在出血热的高发地区一定要加强防鼠、灭鼠与疫苗接种的综合防控措施。
Objective To analyze the viruses carrying of kidney syndrome hemorrhagic fever in host animals in Anshan City from 2017 to 2019,and hantavirus antibody IgG in key population,providing evidence for prevention and control measures.Methods Based on the National Monitoring Program for Hemorrhagic Fever with Renal Syndrome(Trial),immunofluorescence was used to detect rodent lung antigen,enzyme linked immunosorbent(ELISA) was adopted to determine the rodent blood total antibody and key population hantavirus antibody IgG.Results The virus carrying rates in rodents measured by immunofluorescence method were 9.5%,13% and 3% respectively in 2017,2018 and 2019. The virus carrying rates in rodents captured outside the villages were bigger in those captured inside the villages,and the virus carrying rates in rodents captured in spring were bigger than those captured in autumn.The infection rates in rodents determined by ELISA were 16.5%,10% and 6% respectively in 2017,2018 and 2019.The infection rates in rodents captured inside the villages were bigger than those captured outside the villages,and infection rates in rodents captured in autumn were bigger than those captured in spring.The positive rates of antibody IgG in key population determined by ELISA were 7.23%,0 and 12.2% respectively in 2017,2018 and 2019.Conclusion In the areas with high incidence of haemorrhagic fever,comprehensive prevention and control measures against rodents and vaccination must be strengthened.
作者
魏卓超
张巍
邓晓丽
WEI Zhuo-chao;ZHANG Wei;DENG Xiao-li(Anshan Center for Disease Control and Prevention,Anshan 114000,China)
出处
《中华卫生杀虫药械》
CAS
2020年第5期469-472,共4页
Chinese Journal of Hygienic Insecticides and Equipments
基金
2019年辽宁省重点传染病监测项目(鞍财指社[2019]682号)。
关键词
肾综合征出血热
免疫荧光法
酶联免疫吸附实验(ELISA)
带毒率
感染率
hemorrhagic fever with renal syndrome
immunofluorescence method
Enzyme linked immunosorbent assay(ELISA)
virus carrying rate
infection rate
