摘要
目的评价慢性乙型肝炎(CHB)患者外周血中可溶性纤维介素蛋白2(sFGL2)检测的临床意义。方法选取在本院就诊的140例CHB患者作为乙型肝炎病毒感染观察组(CHB组),其中25例CHB患者行抗病毒治疗(抗病毒治疗组);选取同期丙型肝炎患者42例作为丙型肝炎病毒感染观察组(CHC组);选取同期体检健康人群68例(对照组)。酶联免疫吸附试验检测各组血浆sFGL2浓度,实时定量聚合酶链式反应检测各组外周血中sFGL2 mRNA表达,比较它们在各组间的差异;Spearman分析它们与CHB各临床指标的相关性。抗病毒治疗组在抗病毒治疗3月后再次检测血浆sFGL2浓度和外周血中sFGL2 mRNA表达,比较它们抗病毒治疗前后的差异。结果CHB组sFGL2浓度和sFGL2 mRNA表达均高于CHC组和对照组,差异具有统计学意义(P<0.05),而CHC组和对照组sFGL2浓度和sFGL2 mRNA表达比较差异无统计意义(P>0.05)。Spearman相关性分析显示,sFGL2浓度和sFGL2 mRNA表达与HBV-DNA、ALT、AST呈正相关(P<0.05),与GGT、T-Bil、AFP无相关性(P>0.05)。抗病毒治疗后sFGL2浓度和sFGL2 mRNA表达较抗病毒前均显著下降,差异具有统计学意义(P<0.05)。结论CHB患者sFGL2显著升高,它与HBV-DNA载量、ALT、AST异常密切相关,抗病毒治疗后sFGL2显著下降。
Objective To evaluate the clinical value of soluble fibrinogen-like protein 2(sFGL2) in peripheral blood of patients with chronic hepatitis B(CHB).Methods Enzyme-linked immunosorbent assay and real-time fluorescent quantitative PCR were detected the levels of plasma sFGL2 and sFGL2 mRNA in peripheral blood of 140 CHB patients(CHB group),in which 25 cases were treated with antiviral drugs(antiviral group)、42 HCV patients(CHC group)and 48 healthy cases(control group),comparing the differences among those groups;Spearman correlation was analyzed between sFGL2 level and clinical indexes in CHB patients;estimating the differences in the levels of plasma sFGL2 and sFGL2 mRNA before and after antiviral treatment in antiviral group of 25 cases.Results The levels of plasma sFGL2 and sFGL2 mRNA in the CHB group were higher than those in the CHC group and the control group,the difference were statistically significant(P<0.05),no statistical difference between the CHC group and the control group in plasma s FGL2 and s FGL2 mRNA(P>0.05).Spearman correlation analysis showed that sFGL2 concentration and sFGL2 mRNA expression were positively correlated with HBV-DNA,ALT and AST(P<0.05),but not correlated with GGT,T-Bil,and AFP(P>0.05).The concentration of sFGL2 and the expression of sFGL2 mRNA after antiviral treatment were significantly lower than before antiviral treatment,and the difference was statistically significant(P<0.05).Conclusion SFGL2 is significantly increased in CHB patients,which is closely related to the abnormalities of HBV-DNA load,ALT and AST,and sFGL2 is significantly decreased after antiviral treatment.
作者
洪晓绿
潘小平
徐沛演
黄小华
HONG Xiaolv;PAN Xiaoping;XU Peiyan;HUANG Xiaohua(Department of Infection Diseases,Huadu District People's Hospital,Guangzhou,Guangdong,China,510800;Department of Clinical Laboratory,Huadu District People's Hospital,Guangzhou,Guangdong,China,510800)
出处
《分子诊断与治疗杂志》
2020年第10期1307-1310,共4页
Journal of Molecular Diagnostics and Therapy
