摘要
为探讨绵羊血小板糖蛋白V基因(Glycoprotein V,Platelet,GP5)编码蛋白的结构和功能,以NCBI网站的GenBank数据库中发布的绵羊GP5基因序列(登录号为XM_027965957.1)为基础,采用生物信息学在线工具和软件对绵羊的GP5基因进行生物信息学分析。结果表明,绵羊GP5基因序列中包含1个最大长度为1620 bp的开放阅读框,推测其编码539个氨基酸,其中亮氨酸含量最多,为22.6%。GP5基因所编码的蛋白其相对分子质量约为59305.38 KDa,理论等电点为9.40;亚细胞定位结果表明其主要位于内质网(44.4%)。GP5蛋白含有信号肽和跨膜螺旋结构,在二级结构预测中,该蛋白以无规卷曲为主,为70.14%。GP5蛋白三级结构主要由无规卷曲折叠缠绕形成,与二级结构预测结构一致。
In order to investigate the structure and function of the protein encoded by Glycoprotein V,Platelet(GP5),this paper uses bioinformatics online tools and software to analyze the GP5 gene of sheep based on the GP5 gene sequence of sheep published in GenBank database of NCBI website(registration number:XM_027965957.1).The results showed that GP5 gene sequence of sheep contained an open reading frame with a maximum length of 1620 bp,and it was speculated that it encoded 539 amino acids,of which leucine content was the largest(22.6%).The relative molecular weight of the protein encoded by GP5 gene is about 59305.38 KDa,and the theoretical isoelectric point is 9.40.The results of subcellular localization showed that it was mainly located in the endoplasmic reticulum(44.4%).GP5 protein contains signal peptide and transmembrane helix structure.In the prediction of secondary structure,random crimp was dominant,which was 70.14%.The tertiary structure of GP5 protein is mainly formed by random crimp,winding and folding,which is consistent with the predicted structure of the secondary structure.
作者
宋雅萍
李彦霞
郭文婧
张小雪
SONG Yaping;LI Yanxia;GUO Wenjing;ZHANG Xiaoxue(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou Gansu 730070,China)
出处
《甘肃农业科技》
2020年第10期54-59,共6页
Gansu Agricultural Science and Technology
基金
甘肃农业大学学生科研训练计划项目(202004029)。
