摘要
目的观察抗IL-17A单克隆抗体(司库奇尤单抗)治疗前后寻常性银屑病患者血清细胞因子的变化,探讨细胞因子的检测在其疗效评价中的意义。方法收集接受司库奇尤单抗治疗的21例寻常性银屑病患者临床资料,采集外周血,通过流式多因子微珠阵列(cytometric bead array,CBA)检测患者血清中IL-1β、IL-2、IL-5、IL-6、IL-8、IL-10、IL-12p70、IL-17A、IFN-γ浓度。结果寻常性银屑病患者血清中细胞因子IL-8(P<0.01)、IL-17A(P=0.03)与患者PASI评分正相关,司库奇尤单抗治疗后5周患者血清细胞因子IL-2(P<0.05)、IL-17A(P<0.05)水平升高,IL-6水平降低(P<0.05),但IL-1β、IL-5、IL-8、IL-10、IL-12p70、IFN-γ水平的差异无统计学意义(P>0.05)。与治疗前相比,治疗12周后患者血清细胞因子IL-1β(P<0.05)、IL-12p70(P<0.05)水平升高,IL-17A(P<0.05)水平持续上升。同时,原本降低的IL-6水平上升(P<0.05)。司库奇尤单抗治疗后患者皮损改善程度与血清中细胞因子ΔIL-6(P<0.05)、ΔIL-5(P=0.03)呈正相关(P=0.01)。结论在司库奇尤单抗治疗寻常性银屑病早期监测中,血清细胞因子IL-5、IL-6的变化与PASI评分的改善具有显著相关性,可能作为寻常性银屑病患者评估疗效的客观生物标记。
Objective To investigate serum cytokines in psoriasis vulgaris patients before and after IL-17A inhibition by secukinumab.We want to explore whether the detection of cytokines can be used to evaluate the efficacy and sensitivity.Methods Twenty-one patients with psoriasis vulgaris treated with secukinumab were selected.Serum samples were obtained from these patients,and cytometric bead array(CBA)was performed to detect the levels of interleukin(IL)-1β、IL-5、IL-6、IL-8、IL-10、IL-12p70、IL-17A、IFN-γ.Results Serum cytokines IL-8(P<0.05)and IL-17A(P<0.05)were positively correlated with PASI score.After 5-weeks treatment,these patients showed significantly increased serum levels of IL-2(P<0.05),IL-17A(P<0.05),while IL-6(P<0.05)decreased.However,there was no significant difference in the serum level of IL-1β、IL-5、IL-8、IL-10、IL-12p70、IFN-γafter treatment.After 12-weeks treatment,these patients showed significantly increased serum levels of IL-1β(P<0.05),IL-12p70(P<0.05)and IL-17A(P<0.05),IL-6 which was down-regulated after 5weeks treatment,also increased at 12 weeks(P<0.05).The improvement of PASI score was positively correlated withΔIL-5(P<0.05)andΔIL-6(P<0.05)in serum.Conclusion The change of serum IL-5、IL-6 was positively correlated with PASI score,and these cytokines may be used as a biomarker clinically to evaluate the treatment efficacy.
作者
张莉
陈利红
李霞
薛峰
乐云辰
胡梦妍
徐倩楠
郑捷
ZHANG Li;CHEN Lihong;LI Xia;XUE Feng;LE Yunchen;HU Mengyan;XU Qiannan;ZHENG Jie(Department of Dermatology Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200025,China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2020年第11期1249-1254,共6页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金(81830095,81803134)。
