摘要
建立了食用植物油中苯残留量的测定方法。本方法采用顶空-气相色谱法,选择毛细管色谱柱、平衡温度、平衡时间和称样质量分别为DB-624、70℃、30min和5g,以外标法定量。该方法在0.250-40.0mg/kg质量浓度范围内呈线性关系,相关系数为0.9997,检出限为0.08 mg/kg,定量限为0.25 mg/kg。在空白基质中做加标回收试验,添加水平为0.250、5.00和20.0mg/kg,得到平均回收率为94.3-103.7%,相对标准偏差(RSD)范围为2.66-3.75%。该方法操作方便、简单、快捷、清洁、灵敏度高、重复性好,可在大多数分析实验室推广应用,适用于食用植物油中苯残留量的测定。
A method for the determination of benzene residue in edible vegetable oil was established.In this method,headspace-gas chromatography was used,and the capillary column,equilibrium temperature,equilibrium time and sample weight were selected as DB-624,70℃,30min and 5 g,and external standard method was used for quantification.The method presented a linear relationship in the mass concentration range of 0.250 to 40.0 mg/kg,with a correlation coefficient of 0.9997,a detection limit of 0.08 mg/kg,and a quantitative limit of 0.25 mg/kg.The spiked recovery test was performed in a blank matrix with the addition levels of 0.250,5.00,and 20.0 mg/kg.The average recovery was 94.3%to 103.7%,and the relative standard deviation(RSD)range was 2.66%to 3.75%.The method is convenient,simple,fast,clean,sensitive,and repeatable.It can be widely used in most analytical laboratories for the determination of benzene residues in edible vegetable oils.
作者
江丰
朱正伟
王会霞
付文雯
张莉
JIANG Feng;ZHU Zheng-wei;WANG Hui-xia;FU Wen-wen;ZHANG Li(Hubei Provincial Institute for Food Supervision and Test,Wuhan Hubei 430075,China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test,Wuhan Hubei 430075,China)
出处
《食品与发酵科技》
CAS
2020年第5期118-122,127,共6页
Food and Fermentation Science & Technology
基金
国家市场督管理总局食品补充检验方法项目(BJS201906)。
关键词
食用植物油
苯
顶空-气相色谱
edible vegetable oil
benzene
headspace-gas chromatography
