摘要
目的:通过对0~1岁的婴幼儿接种了乙肝疫苗后2周内检测HBsAg的统计研究,阳性率接近10%,分析HBsAg低滴度阳性,且阳性率比较高是否和接种疫苗相关,并探讨其机理及临床意义。方法:选取本院2016年至2019年儿科病房及免疫门诊0~1岁接种乙肝疫苗的婴幼儿,接种后2周内用化学发光微粒子法检测HBsAg,检出HBsAg低滴度阳性的193例血清标本进行研究,并对这193例0~1岁婴幼儿半年后同样检测方法再复检HBsAg,统计阳性率,并对医院常用的不同批次的3支疫苗进行不同浓度稀释后用同样检测方法检测HBsAg,统计阳性结果的稀释倍数,再通过肌注疫苗后理论推算疫苗在不同阶段的婴幼儿体内的浓度,对不同类别检测结果进行统计学分析。结果:193例婴幼儿半年以后复检HBsAg,4例阳性,阳性率仅为2.1%,说明0~1岁接种乙肝疫苗的婴幼儿2周内检测HBsAg低滴度高阳性率和接种疫苗有直接关系,差异有统计学意义(P<0.05);对不同浓度疫苗HBsAg检测结果和疫苗在不同阶段婴幼儿体内浓度分析,疫苗在体内稀释浓度应该表现为阴性结果,而HBsAg低滴度高阳性率的原因可能为疫苗刺激机体产生了免疫效应有关,这种差异有统计学意义(P<0.05)。结论:接种了乙肝疫苗的0~1岁婴幼儿出现HBsAg低滴度高阳性率和疫苗接种有相关性,大部分婴幼儿半年以后检测HBsAg转阴,有少部分未转阴的可能是和疫苗接种无关,是曾经感染了的HBV成为了乙肝携带者,通过以上研究分析,可为大夫对儿童是否感染乙肝的临床诊断提供理论依据!
Objective:Through the statistical study on the detection of HBsAg in 0~1 year-old infants within 2 weeks after vaccination,the positive rate was close to 10%.To analyze whether the low titer of HBsAg was positive and the positive rate was relatively high,whether it was related to vaccination,and to explore its mechanism and clinical significance.Methods:Children aged 0~1 years who were vaccinated with hepatitis B vaccine in pediatric ward and immune clinic from 2016 to 2019 were selected.The serum samples of 193 cases with low HBsAg titer positive were detected by chemiluminescent microparticle method within 2 weeks after vaccination.The same detection method was used to retest the HBsAg of these 193 infants aged 0~1 years after half a year,and the positive rate was counted After the vaccine was diluted with different concentrations,the same detection method was used to detect HBsAg,the dilution multiple of positive results was counted,and then the concentration of vaccine in different stages of infants was calculated by intramuscular injection,and the detection results of different types were statistically analyzed.Results:In 193 infants,HBsAg was re-examined half a year later.Four cases were positive,with a positive rate of only 2.1%,indicating that the low titer of HBsAg detected within 2 weeks in 0~1 year old infants with hepatitis b vaccine was directly related to vaccination,and the difference was statistically significant(P<0.05),HBsAg vaccine in different concentration,test results and vaccines in the different stages of infant concentration analysis of vaccine to dilute concentration in the body should be characterized by negative result,the cause of the high positive rate and low HBsAg drops degrees may be related to stimulate the body to produce the vaccine immune effect,the difference have statistical word meaning(p<0.05).Conclusion:the hepatitis b vaccine inoculation of 0 and 1 years old infants and young children appear HBsAg.Low-grade high positive rate and correlated with vaccination,most of the i
作者
安和兵
陈珊
马媛媛
张俊婷
王书旺
杨建宝
杨敬芳
AN Hebing;MA Yuanyuan(The Second Affiliated Hospital of Hebei North University,Hebei Zhangjiakou 075100,China)
出处
《河北医学》
CAS
2020年第10期1698-1701,共4页
Hebei Medicine
基金
河北省张家口市科技攻关计划项目,(编号:1621032D)。
关键词
儿童
乙肝疫苗
HBSAG
微粒子法
Children
Hepatitis
B vaccine
HBsAg
Microparticle method
