血必净注射液对缺氧-复氧大鼠心肌炎性反应中白细胞介素-1β水平的影响被引量：1

Xuebijing Injection Has Effect on Interleukin-1 β Level in Myocardial Inflammatory Response in Hypoxia-Reoxygenation Rats

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摘要目的:探讨血必净注射液(XBJI)对缺氧-复氧大鼠心肌炎性反应中白细胞介素-1β(IL-1β)水平的影响。方法:选用雄性SD大鼠48只,随机分为对照组、平衡灌注组、模型组、XBJI低剂量组、XBJI中剂量组、XBJI高剂量组6组,每组8只。采用大鼠离体心脏经主动脉行恒温恒压灌注,经灌注/停灌/再复灌的方式建立大鼠心肌缺氧-复氧模型。XBJI低剂量组、XBJI中剂量组、XBJI高剂量组在大鼠心肌缺氧-复氧模型制备中分别加入20 mL/L、40 mL/L、80 mL/L的XBJI。检测心肌组织中IL-1β蛋白及mRNA与心肌中肌酸激酶同工酶(CK-MB)的浓度。结果:与对照组比较,平衡灌注组心肌组织IL-1β mRNA相对含量上升(P<0.05);CK-MB、IL-1β蛋白浓度比较,差异无统计学意义(P>0.05)。与平衡灌注组比较,模型组心肌组织CK-MB、IL-1β蛋白浓度及IL-1β mRNA相对含量均升高(P<0.05)。与模型组比较,XBJI各剂量组心肌组织CK-MB、IL-1β蛋白浓度均下降(P<0.05);XBJI中剂量组心肌组织IL-1β mRNA相对含量下降(P<0.05)。与XBJI中剂量组比较,XBJI低、高剂量组CK-MB蛋白浓度较高(P<0.05),XBJI高剂量组IL-1β蛋白浓度较高(P<0.05)。结论:XBJI可抑制心肌CK-MB活性,降低IL-1β水平,减轻缺氧-复氧大鼠心肌的炎性反应,且中剂量效果最佳。 Objective:To discuss the effect of Xuebijing injection(XBJI) on the level of interleukin-1β(IL-1β) in myocardial inflammatory response in rats after hypoxia-reoxygenation. Methods:A total of 48 male SD rats were selected and randomly divided into the control group,the balanced perfusion group,the model group,and the XBJI groups of low-dose,mediumdose and high-dose,eight rats in each group. The isolated rat hearts were treated with constant temperature and pressure perfusion via aorta;after perfusion for twice,the rat models with myocardial hypoxia and reoxygenation were established. In the XBJI groups of low-dose,medium-dose and high-dose,during the establishment of rat models,20,40 and 80 mL/L XBJI was added respectively. The concentrations of protein and mRNA of IL-1β and creatine kinase isoenzyme(CK-MB) in myocardium were detected. Results: There was no significant difference being found in the comparison of the protein concentrations CK-MB and IL-1β in myocardium between the control group and the balanced perfusion group(P>0.05),and the relative content of mRNA of IL-1β was increased(P<0.05). Compared with those in the balanced perfusion group,the protein concentrations of CK-MB and IL-1β and the relative content of mRNA of IL-1β in myocardium in the model group were increased(P<0.05). Compared with those in the model group,the protein concentrations of CK-MB and IL-1β in the XBJI groups of different dosages were all decreased(P<0.05),and the relative content of mRNA of IL-1β in myocardium was decreased in the group of medium-dose(P<0.05). Compared with those in the XBJI group of medium-dose, the protein concentrations of CK-MB in the groups of low-dose and high-dose were higher(P<0.05),and the protein concentration of IL-1β in the XBJI group of high-dose was higher(P<0.05). Conclusion:XBJI can inhibit the activity of CK-MB,reduce the level of IL-1β and alleviate myocardial inflammatory response in rats after hypoxia and reoxygenation. The medium dose of XBJI has the best effect.