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中国传统发酵乳中嗜热链球菌H2的培养及益生特性评价 被引量:4

Cultivation of Streptococcus thermophilus H2 and the Evaluation on Its Prebiotic Properties in Chinese Traditional Fermented Milk
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摘要 为促进国产乳品发酵剂自主研发,从内蒙传统发酵乳中分离鉴定一株嗜热链球菌菌株H2(Streptococcus thermophilus),通过响应面法优化培养基组成,采用体外分析法对菌株H2进行益生特性评价。结果表明,培养基氮源为45.25 g/L酵母提取物、生长因子为169.5 mg/L半胱氨酸、缓冲体系补充物为0.705 g/L碳酸钙且培养条件控制为温度40℃、初始pH值5.5、接种量3.5%时,培养液中活菌数达4.20×10^9 CFU/mL,是优化前的37.8倍。体外益生特性评价实验证明,H2在pH=3时存活率为49.65%,胆盐质量分数为0.3%时存活率可达99.62%。H2的发酵上清液经高效液相色谱(HPLC)法检测,分别检测到L-乳酸、甲酸、乙酸、丙酸、异丁酸、异戊酸等多种发酵代谢产物,其中L-乳酸浓度达到最大,为12.64 mmol/L,且未检到D-乳酸。H2的发酵上清液对3种自由基均有清除作用,其中对1,1-二苯基-2-三硝基苯肼(DPPH)的清除率最高,为94.06%。 To promote the independent research and development of domestic dairy starter,Streptococcus thermophilus H2 was isolated from Inner Mongolia traditional fermented milk.The composition of culture medium was optimized by response surface methodology,and the probiotic characteristics in vitro of strain H2 were evaluated by in vitro analysis.The results show that,the number of viable bacteria can reach 4.20×10^9 CFU/mL,37.8 times larger than that before optimization,when the nitrogen source is 45.25 g/L yeast extract,growth factor is 169.5 mg/L cysteine,buffer system supplement is 0.705 g/L calcium carbonate,and the culture temperature is 40℃,initial pH value is 5.5,inoculum size is 3.5%.Evaluation of the probiotic characteristics in vitro show that the survival rate of Streptococcus thermophilus H2 is 49.65%when pH is 3 and 99.62%when bile salt is 0.3%.Many kinds of fermentation metabolites,such as L-lactate,formic acid,acetic acid,propionic acid,isobutyric acid and isovaleric acid,were detected by high performance liquid chromatography(HPLC)in fermentation supernatant of H2.The concentration of L-lactate is up to 12.64 mmol/L,and D-lactate is not detected.The supernatant of H2 has scavenging effect on three free radicals,of which the scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)was the highest,reaching 94.06%.
作者 刘冬梅 胡金双 黄泳尧 黄燕燕 LIU Dongmei;HU Jinshuang;HUANG Yongyao;HUANG Yanyan(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China)
出处 《华南理工大学学报(自然科学版)》 EI CAS CSCD 北大核心 2020年第9期133-140,148,共9页 Journal of South China University of Technology(Natural Science Edition)
基金 国家自然科学基金资助项目(31771908) 广东省科技计划项目(2014A020208019) 广州市科技计划项目(201903010015)。
关键词 嗜热链球菌 培养基优化 响应面法 体外益生性质 Streptococcus thermophilus culture medium optimization response surface methodology prebiotic properties
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