摘要
目的评价该中心脊髓灰质炎(简称脊灰)实验室所用细胞系的质量控制情况,保障该市脊灰实验室的脊灰病毒病原学检测质量。方法采用聚合酶链反应对L20B细胞系和RD细胞系培养液进行支原体检测,确定细胞系是否被支原体污染。使用微量96孔细胞培养板对RD细胞系和L20B细胞系进行细胞敏感性实验。结果在2013-2019年,该市脊灰实验室使用的细胞系支原体检测结果均为阴性,3个型别的脊灰病毒在RD细胞系和L20B细胞系上的细胞敏感性实验结果都在正常范围内波动。结论该市脊灰实验室使用的细胞系质量稳定,能满足脊灰病毒检测敏感性的要求。脊灰实验室的细胞系质量控制具有重要意义,在细胞系质量控制中发现问题必须及时解决,以保证脊灰病毒分离工作高质量地运行。
Objective To evaluate the quality control situation of the cell lines used in the poliomyelitis laboratory of this center for ensuring the quality level of pathogenic detection of poliovirus in Chongqing poliomyelitis laboratory.Methods The polymerase chain reaction(PCR)method was used to conduct the mycoplasma detection in culture medium of L20B cell line and RD cell line to determine whether the cell lines were contaminated by mycoplasma.The RD cell and L20B cell lines conducted the cellular sensitivity test by using microcells 96-well culture plates.Results From 2013 to 2019,all the cell lines mycoplasma detection results were negative.The cellular sensitivity results of the three serotypes of poliovirus on RD and L20B cell lines fluctuated within the normal range.Conclusion The quality of the cell lines used in the Chongqing poliomyelitis laboratory is stable,which can meet the sensitivity of poliovirus detection.The quality control of cell lines in the poliomyelitis laboratory is of great significance.The problems found in quality control of cell lines must be resolved in time to ensure the high quality operation of poliovirus isolation work.
作者
彭靖尧
黄为
谢武娟
凌华
张敏
赵华
PENG Jingyao;HUANG Wei;XIE Wujuan;LING Hua;ZHANG Min;ZHAO Hua(Chongqing Municipal Center for Disease Control and Prevention,Chongqing 400042,China)
出处
《检验医学与临床》
CAS
2020年第20期2929-2932,共4页
Laboratory Medicine and Clinic
基金
重庆市科学技术委员会和重庆市卫生健康委员会合医学科研项目(2018MSXM077)。
