摘要
目的监测北京市丰台区环境污水中的肠道病毒的血清型分布及流行情况，为北京市丰台区肠道病毒相关的传染病预防和控制提供实验室支持。方法选择吴家村污水处理厂为监测点，2012年每月采集污水（共12份），用阴离子膜一碱洗脱法进行病毒浓缩，然后进行病毒分离，并对阳性分离物进行肠道病毒血清型鉴定。结果北京市丰台区环境监测污水标本中共分离到60株肠道病毒、6株腺病毒；病毒分离以B组肠道病毒为主；夏季（5—9月）的病毒分离率较高；部分环境中分离的肠道病毒与手足口患者体内标本有同源性。结论环境监测可以为北京市丰台区肠道病毒病例监测系统提供重要的补充信息。本研究可以为丰台区乃至北京市的EV研究提供背景信息。
Objective To provide laboratory data for the prevention and control of the human entero- virus infection diseases in Fengtai district of Beijing, human enterovirus in enviromental sewage was moni- tored. Methods The Wu-jia-cun sewage treatment plant was chose as surveillance site, the sewage samples were collected monthly from in Fengtai district, from January 2012 to December 2012. Viral isolation was performed for the sewage samples. Results 60 enteroviruses and 6 adenoviruses were isolated from sewage water samples, HEV-B were the mainly enteroviruses during the environmental surveillance. Virus separation rate is higher in May, Jun, Jul, Aug and Sep. Part of the enterovirus isolated from environment specimens have homology with hand-foot-mouth of patients specimens. Conclusions Environmental surveillance can provide important supplementary information for enteroviruses case surveillance system which could provides background information on the EV study for the Fengtai district.
出处
《国际病毒学杂志》
2014年第5期205-208,共4页
International Journal of Virology
基金
国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项：重大传染病应急处置检测技术平台（编号：2012ZX10004101）
中共北京市委、北京市政府重点工作及区（县）政府应急项目预启动（编号：Z111107056811042）
关键词
污水
肠道病毒
监测
病毒分离
Sewage
Enterovirus
Surveillance
Viral isolation
