摘要
目的对埃可病毒6型(ECHO6)福建龙岩分离株进行分子生物学分析,阐明ECHO6病毒福建龙岩分离株的分子生学特征及其与世界其它分离株的基因关系。方法采集暴发病例脑脊液标本,用RD细胞进行分离病毒,阳性分离物采用中和试验及PCR序列分析确定其血清型别;RT-PCR扩增VP1基因全长,测定序列进行同源性及亲缘进化树分析。结果 13例细胞培养阳性分离物经中和试验及序列分析结果均为ECHO6。随机选取4株病毒株进行VP1全基因序列分析显示:4个病毒株之间同源性>99%;与山东病毒株E6/SD/sewage/081226/2-2同源性高于其他参考株。在遗传进化树上,4株分离病毒株同属一个分支,为C2亚型。结论 ECHO6是引起本次福建省龙岩市长汀县儿童病毒性脑炎暴发的病原体,其基因型为C2亚型。
The molecular biologicai analysis method was used to clarify molecular characteristics of the echovirus type 6 （ECHO6） virus isolated in Longyan City in Fujian and to recognize its relationship with the isolates in the rest of world. The virus were isolated from patientg cerebrospinal fluid （CSF） specimens with rhabdomyosarcoma （RD） cells, and the serotypes were classified through neutralizing test on positive isolates during the outbreak of children viral encephalitis in Changting County in Fujian Province, May of 2010. The complete VP1 sequences of the 4 isolates in I.ongyan were successfully amplified and compared with other ECHO6 isolates available from GenBank, and then they were constructed a VP1 gene phylogenetic tree. In 4 sequenced strains, the length of VP1 coding region was 866 bp （1 strain） and the others were 867 bp （3 strains）. The homologies of these 4 strains were higher than 99%o. The VP1 nucleotide sequence homology matrix analysis showed that 4 strains of the virus had the highest homology with Shandong virus strain E6/SD/sewage/081226/2 2. Phylogenetic analysis showed that these four ECHO6 strains were in the same branch of C2 genotype. In conclusion, ECHOG was the pathogen of this children viral encephalitis outbreak in Changting; Fujian, and the genotype is C2 subtype.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第5期523-526,共4页
Chinese Journal of Zoonoses
关键词
病毒性脑炎
埃可病毒6型
序列分析
基因特征
viral encephalitis
ECHO 6
sequence analysis
genetic characterization
