摘要
背景:低氧暴露下体质量的减少被认为与摄食量减少有关,胃饥饿素(Ghrelin)水平下降能使摄食量减少,但是肥胖所致消化系统营养吸收障碍和血脂异常能否在间歇低氧干预下通过Ghrelin-GHSR通路进行调控,目前尚不清楚。目的:明确间歇低氧暴露对肥胖小鼠胃组织Ghrelin-GHSR通路的影响。方法:将30只离乳C57BL/6J小鼠随机分为普通对照组(n=8)和高脂膳食组(n=22)。高脂膳食组饲喂8周高脂饲料建立肥胖模型后随机分为肥胖对照组(n=8)和肥胖低氧组(n=8),剩余体质量未超过普通对照组平均体质量20%的小鼠弃去。肥胖低氧组进行4周低氧暴露(氧浓度为11.2%),8 h/d,6次/周。干预结束后检测血清生化和Ghrelin水平,RT-PCR测定胃组织Ghrelin和下丘脑生长激素促分泌激素受体1a mRNA相对表达水平,苏木精-伊红染色法观察胃组织形态,免疫组化法测定胃组织Ghrelin和Ghrelin O-酰基转移酶(Goat)的平均吸光度值,Western Blot测定胃Ghrelin和Ghrelin O-酰基转移酶、下丘脑生长激素促分泌激素受体1a蛋白表达水平。实验方案经北京体育大学运动科学伦理委员会批准(审批号:2015021)。结果与结论:①低氧干预4周使肥胖对照组体质量显著高于普通对照组和肥胖低氧组;②肥胖对照组血糖、总胆固醇、三酰甘油水平显著高于普通对照组,肥胖低氧组血糖、总胆固醇水平显著低于肥胖对照组,肥胖低氧组血清Ghrelin水平显著高于肥胖对照组;③肥胖对照组胃组织Ghrelin mRNA相对表达量显著低于肥胖低氧组,下丘脑生长激素促分泌激素受体1a mRNA水平极显著低于普通对照组;④肥胖低氧组胃底腺主细胞规律、密集、均匀分布;⑤肥胖低氧组Ghrelin和Ghrelin O-酰基转移酶的平均吸光度值、生长激素促分泌激素受体1a、Ghrelin和Ghrelin O-酰基转移酶蛋白含量显著高于肥胖对照组;⑥结果说明,4周低氧暴露可显著上调肥胖小鼠胃组织Ghre
BACKGROUND:Weight loss under hypoxic exposure is related to a reduction in food intake.Decreased gastric Ghrelin levels can reduce food intake,but whether digestive nutritional absorption disorders and dyslipidemia caused by obesity can be regulated through Ghrelin-growth hormone secretagogue receptor(GHSR)pathway under intermittent hypoxia is unclear yet.OBJECTIVE:To explore the effect of intermittent hypoxia exposure on Ghrelin-GHSR pathway in gastric tissues of obese mice.METHODS:Thirty C57BL/6J male mice were randomly divided into a control group(n=8)and a high fat diet group(n=22,fed with high fat diet).After 8 weeks high fat diet,obesity models were successfully established in 16 mice with high fat diet,and then were randomly divided into an obese control group(n=8)and an obese hypoxic group(n=8,11.2%oxygen concentration,8 hours per day,6 times per week).The mice whose remaining body mass did not exceed the average body weight of the normal control group by 20%were discarded.After intervention,the serum biochemical indicators and Ghrelin level were tested;the Ghrelin and GHSR-1a mRNA expressions were tested by RT-PCR;gastric tissue morphology was observed by hematoxylin-eosin staining;the mean absorbance values of Ghrelin and Ghrelin O-acyltransferase(Goat)were measured by immunohistochemistry;the protein expressions of hypothalamic growth hormone secretagogue hormone receptor 1a(GHSR-1a),Ghrelin and Goat were detected by western blot.The study protocol was approved by the Sports Science Ethics Committee of Beijing Sport University,approval No.2015021.RESULTS AND CONCLUSION:(1)The body mass of the obese control group was significantly higher than that of the control group and obese hypoxic group after 4-week hypoxic intervention.(2)The blood glucose,total cholesterol,and triacylglycerol levels were obviously higher in the obese control group than the control group.Compared with the obese control group,the blood glucose and total cholesterol levels were significantly lower in the obese hypoxic group,while th
作者
杨璐瑶
付鹏宇
唐舒宁
朱镕鑫
龚丽景
Yang Luyao;Fu Pengyu;Tang Shuning;Zhu Rongxin;Gong Lijing(China Institute of Sport and Health,Beijing Sport University,Beijing 100084,China;School of Sports and Human Sciences,Beijing Sport University,Beijing 100084,China;Shanghai Research Institute of Sports Science,Shanghai 200030,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第11期1733-1739,共7页
Chinese Journal of Tissue Engineering Research
基金
北京市大学生创新创业训练计划项目(201910043040),项目负责人:杨璐瑶。
关键词
低氧
肥胖
胃组织
下丘脑
胃饥饿素
生长激素促分泌素受体
hypoxia
obesity
gastric tissue
hypothalamus
Ghrelin,growth hormone secretagogue receptor
