摘要
目的:建立鸡冠花药材HPLC指纹图谱测定方法,对市售鸡冠花药材的质量进行较全面的评价。方法:采用Waters HSS T3色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸溶液(含0.2%三乙胺)为流动相进行梯度洗脱,指纹图谱检测波长为290 nm,山柰素、异鼠李素、山奈酚-4’-O-甲醚含量测定检测波长为365 nm,柱温为30℃,流速为1.0 mL/min。采用相似度评价、聚类分析对15批鸡冠花药材指纹图谱进行研究。结果:建立了鸡冠花药材的HPLC指纹图谱,确定了8个共有峰;通过相似度评价、聚类分析结果可知15批鸡冠花药材质量差异不明显,并比较了样品中山柰素、异鼠李素、山奈酚-4’-O-甲醚的含量。结论:该方法为鸡冠花药材的质量控制提供了较为全面、有效的快速评价方法。
Objective: To establish a method for the determination of HPLC fingerprint of Jiguanhua(celosiae cristatae flos), and to evaluate the quality of commercial Jiguanhua(celosiae cristatae flos). Methods: A Waters HSS T3 column(4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.2% phosphoric acid solution(containing 0.2% triethylamine) as the mobile phase for gradient elution. The detection wavelength of fingerprint was 290 nm, and the detection wavelength of kaempferol, isorhamnetin and kaempferol-4 ’-o-methyl ether was 365 nm.The column temperature was 30 ℃, and the flow rate was 1.0 mL/min. The fingerprints of 15 batches of Jiguanhua(celosiae cristatae flos) were studied by similarity evaluation and cluster analysis. Results: The HPLC fingerprint of Jiguanhua(celosiae cristatae flos) was established, and 8 common peaks were determined. The results of similarity evaluation and cluster analysis showed that the quality of 15 batches of Jiguanhua(celosiae cristatae flos) was not significantly different, and the contents of kaempferin, isorhamnetin and kaempferol-4’-O-methyl ether in the samples were compared. Conclusion: This method provides a more comprehensive and effective rapid evaluation method for the quality control of Jiguanhua(celosiae cristatae flos).
作者
李国卫
孙冬梅
何民友
吴淑珍
吴文平
曹斯琼
LI Guo-wei;SUN Dong-mei;HE Min-you;WU Shu-zhen;WU Wen-ping;CAO Si-qiong(Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan Guangdong 528244,China)
出处
《中医药导报》
2020年第13期64-67,71,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省科技厅课题(2017TY04R197):广东省“特支人才计划”科技创业领军人才项目
广东省中药配方颗粒企业重点实验室(2018B030323004)。
关键词
鸡冠花
指纹图谱
黄酮
山柰素
异鼠李素
山奈酚-4’-O-甲醚
聚类分析
Jiguanhua(celosiae cristatae flos)
fingerprint
flavonoids
kaempferin
isorhamnetin
kaempferol-4’-O-methyl ether
cluster analysis
