摘要
目的应用Micro-CT技术观察淫羊藿苷和朝藿定C对糖皮质激素性骨质疏松症(GIOP)小鼠模型骨组织骨量、骨微结构的影响。方法8周龄C57/BL6雄性小鼠随机分为4组:对照组、模型组、淫羊藿苷(200 mg/kg)组和朝藿定C(200 mg/kg)组,除对照组外,其他3组小鼠im地塞米松5 mg/kg,0.125 mg/次,每周3次,制备GIOP模型,对照组im等体积的生理盐水,造模同时,每天ig给药1次,连续60 d后取材,采用micro-CT方法对胫骨近端骨微结构进行三维分析;HE染色病理切片观察胫骨近端骨组织病理形态。结果骨量参数:与对照组比较,模型组骨矿物质含量(BMC)、骨矿物质密度(BMD)、组织矿物含量(TMC)、组织矿物质密度(TMD)均显著下降(P<0.05、0.01);与模型组比较,淫羊藿苷组、朝藿定C组BMC、BMD、TMC、TMD指标均显著提高(P<0.05);其中朝藿定C组各指标均较淫羊藿苷组显著升高(P<0.05)。与对照组比较,模型组相对骨体积(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)指标均显著下降(P<0.05),骨小梁分离度(Tb.Sp)、结构模型指数(SMI)均显著提高(P<0.05);与模型组比较,淫羊藿苷、朝藿定C组BV/TV、Tb.N、Tb.Th显著升高,Tb.Sp、SMI显著下降(P<0.05),其中朝藿定C组各指标均较淫羊藿苷组改善显著(P<0.05)。HE染色病理切片示,模型组骨小梁数目明显减少,稀疏断裂,大部分不能连接成网状,骨髓腔明显增大,骨小梁结构出现较大的空白区域;淫羊藿苷及朝藿定C组小鼠骨小梁明显宽厚,数目也显著增加,骨小梁断裂较少,骨小梁光滑,接近对照组,其中朝藿定C组增加较淫羊藿苷组明显。结论地塞米松诱导的骨质疏松小鼠模型成功建立,朝藿定C和淫羊藿苷抗骨质疏松活性明显,主要通过增加骨量和改善骨小梁微结构来最终提高骨强度,其中朝藿定C抗骨松作用更强;Micro-CT技术与传统的检测方法相比,在中药干预GIOP骨微结构参数分析上具有便捷、高效,经济,图像多
Objective Micro-CT technique was used to observe the effects of icariin and epimedin C on bone mass and bone microstructure in the mouse model of glucocorticoid osteoporosis(GIOP).Methods Tatolly 40 C57/BL6 male mice of 8-week-old were randomly divided into four groups:control group,model group,icariin(200 mg/kg)group,and epimedin C(200 mg/kg)group.Except the control group,the other three groups were injected with dexamethasone 5 mg/kg,0.125 mg/time,three times a week,and the control group was injected with physiological saline of equal volume.At the same time,the drugs were gave by gavage once a day,and the samples were taken after 60 days.The microstructure of the proximal tibia was analyzed using Micro-CT method.The histopathology of the proximal tibia was observed by HE staining.Results Bone mass parameters:Compared with the control group,the BMC,BMD,TMC,and TMD indexes of the model group decreased significantly(P<0.01).Compared with the model group,the BMC,BMD,TMC and TMD indexes of the icariin group and the epimedin C group increased significantly.The index of epimedin group C was higher than that of icariin group.Bone microstructure parameters:compared with the control group,the indexes of BV/TV,TB.N,TB.SP and SMI in the model group decreased significantly,TB.SP and SMI increased significantly(P<0.05).Compared with the model group,the indexes of BV/TV,TB.N and TB.Th in the icariin group and epimedin C group increased,and the indexes of TB.SP and SMI decreased significantly(P<0.05).After HE staining pathological section in the model group,the number of bone trabeculae was significantly reduced,with sparse and broken pattern;most of them could not be connected into a network;the bone marrow cavity was significantly increased;the bone trabeculae structure appeared a large blank area.In the icariin and epimedin C group,similar to the control group,the bone trabeculae were obviously wider and thicker;the number was also noticeably increased;the bone trabeculae were less broken;the bone trabeculae were smooth.Mo
作者
刘亚林
黄觅
冯晶
夏平
王瑛
魏孝义
邱玲
LIU Yalin;HUANG Mi;FENG Jing;XIA Ping;WANG Ying;WEI Xiaoyi;QIU Ling(Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Wuhan Hospital of Integrated Traditional Chinese and Western Medicine,Wuhan 430022,China;Guangdong Provincial Key Laboratory of Applied Botany,Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China;Gannan Normal University,Ganzhou,341000,China)
出处
《药物评价研究》
CAS
2020年第9期1733-1739,共7页
Drug Evaluation Research
基金
湖北省自然科学基金资助项目(2018CFB695)
广东省应用植物学重点实验室开放课题(AB2018027)
中国科学院华南农业植物分了分析与遗传改良重点实验室开放课题(KF202002)。
