摘要
[目的]优化CO2超临界萃取孢子油后的破壁灵芝孢子粉残渣和破壁灵芝孢子粉中三萜类成分提取工艺以及CO2超临界萃取孢子油前后三萜类成分的含量差异。[方法]通过设计正交试验,采用紫外-可见分光光度法测量提取液的吸光度,计算提取率,根据正交数据分析,确定最佳提取方案以及含量差异。[结果]对于CO2超临界萃取灵芝孢子油后的破壁灵芝孢子粉来说,最佳提取工艺为以无水乙醇作为提取溶剂,料液比为1∶30(g∶mL),提取时间为2 h,提取温度为70℃,提取次数为4次时提取率最高;对于破壁灵芝孢子粉来说,最佳提取工艺为以无水乙醇为提取溶剂,料液比为1∶20,提取时间为1.5 h,提取温度为50℃,提取次数2次时提取率最高;且二者三萜类成分含量存在于有明显差异。[结论]该研究为进一步分析破壁灵芝孢子粉在CO2超临界萃取孢子油前后三萜类成分含量差异提供理论依据和参考。
[Objective]To optimize the extraction process of triterpenoids from the residues of spore powder of Ganoderma lucidum and spores of Ganoderma lucidum after CO 2 supercritical extraction of spore oil and the difference of triterpenoids content before and after CO 2 supercritical extraction of spore oil.[Method]By designing an orthogonal experiment,the absorbance of the extract was measured by UV-vis spectrophotometry,the extraction rate was calculated,and the optimal extraction scheme and content difference were determined based on the orthogonal data analysis.[Result]The best extraction process for the broken Ganoderma lucidum spore powder after CO 2 supercritical extraction of Ganoderma lucidum spore oil:absolute ethanol as the extraction solvent,the solid-liquid ratio was 1∶30,and the extraction rate was highest when the temperature was 70℃for 2 h and the number of times was 4 times.For Ganoderma lucidum spore powder,the best extraction process was:using absolute ethanol as the extraction solvent,the solid-liquid ratio was 1∶20,the extraction time was 1.5 h,the extraction temperature was 50℃,and the extraction rate is the highest;and the content of triterpenoids in the two was significantly different.[Conclusion]The study provides a theoretical basis and reference for further analysis of the content difference of triterpenes in the broken Ganoderma lucidum spore powder before and after CO 2 supercritical extraction of spore oil.
作者
温艳艳
张静
朱雅琴
周俊
方玲
彭灿
WEN Yan-yan;ZHANG Jing;ZHU Ya-qin(School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230012;Anhui Provincial Key Laboratory of Traditional Chinese Medicine Compounds,Hefei,Anhui 230012;Collaborative Innovation Center for Quality Improvement of Traditional Chinese Medicine in Anhui Province,Hefei,Anhui 230012;Institute of Pharmaceutical Preparations,Anhui Academy of Chinese Medical Sciences,Hefei,Anhui 230012)
出处
《安徽农业科学》
CAS
2020年第19期182-185,188,共5页
Journal of Anhui Agricultural Sciences
基金
安徽省科技重大专项(17030801015)。
关键词
破壁灵芝孢子粉
提取工艺优化
含量测定
方法学考察
正交试验设计
紫外-可见分光光度法
Broken Ganoderma lucidum spore powder
Optimization of extraction process
Content determination
Methodological investigation
Orthogonal experimental design
Ultraviolet-visible spectrophotometry
