摘要
目的分析梅州市2017~2019年外环境禽流感标本检测结果,为预防和控制禽流感疫情提供合理依据。方法收集梅州市2017年1月~2019年12月禽类市场外环境标本4176份,采用荧光定量PCR方法对甲型流感病毒核酸以及H5、H7、H9亚型流感病毒核酸进行检测,依据检测结果分析禽流感病毒污染情况。结果2017~2019年梅州市禽类市场外环境标本禽流感核酸病毒检测中,2018年甲型流感病毒检出数比例呈现上涨趋势,高于2017、2019年,其中H5、H9类型呈现上涨,H7检出率呈现下降,其中案板台面、刀具砧板、禽类粪便、秤具秤盘外环境标本中甲型流感病毒各亚型检出数显著偏高。结论梅州市禽类市场外环境检出多种亚型禽流感病毒,以屠宰、销售环境相关外环境污染状况严重,重点加强对禽类市场外环境清洁消毒制度的落实,对重点人群做好防护工作。
Objective To analyze the test results of avian influenza specimens in external environment of Meizhou′s poultry market during the period from 2017 to 2019,provide reasonable basis on the disease prevention and control.Methods From January 2017 to December 2019,4176 specimens from external environment of Meizhou′s poultry market were obtained.Fluorescent quantitative PCR was applied to detect nuclein acid,H5,H7 and H9 subtypes of avian influenza A virus.Environmental pollution conditions were examined.Results Test results indicated that the detection rate of avian influenza A virus in 2018 was higher than that in 2017 and 2019.H5 and H9 infection rate was increased while H7 rate was decreased.The cases with virus subtypes from chopping board countertops,knife sticky boards,poultry manure,weighing pans were substantially increased.Conclusion Many virus subtypes are generated in the external environment of Meizhou poultry market,especially for those of animal slaughter and selling booths.Therefore,the disinfection system of poultry market environment shall be implemented for protection of focus groups.
作者
邹勇文
陈志青
ZOU Yong-wen;CHEN Zhi-qing(Meizhou City Disease Prevention and Control Center,Guangdong Province,Meizhou514000,China)
出处
《中国当代医药》
2020年第27期247-249,共3页
China Modern Medicine
关键词
梅州市
外环境
禽流感
标本
检测
Meizhou city
External environment
Avian influenza
Specimens
Detection
