摘要
目的了解淄博地区血小板捐献者人类白细胞抗原(human leukocyte antigen,HLA)A,B位点和人类血小板抗原(human platelet antigen,HPA)1-17系统基因多态性分布特点,初步建立淄博地区血小板捐献者已知HLA、HPA基因型资料库。方法分别用聚合酶链反应-碱基序列直接测序(polymerase chain reaction-sequencing based typing,PCR-SBT)和聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primer,PCR-SSP)方法对200名无血缘关系的单采血小板捐献者进行HLA-A,B和HPA1-17系统基因分型,计算基因频率、基因型频率。结果共检出HLA-A位点低分辨等位基因15个,HLA-B位点低分辨等位基因29个,其中HLA-A位点基因频率居前五位的是A^*02、A^*24、A^*11、A^*30、A^*33,基因频率分别为0.2600、0.1725、0.1375、0.1350、0.0700;HLA-B位点基因频率居前五位的是B^*13、B^*15、B^*40、B^*44、B^*51,基因频率分别为0.1600、0.1325、0.1300、0.0675、0.0625。HPA1-17中,HPA4a、7a-14a、16a、17a呈单特异性,未检测出相应的对偶基因抗原HPA-b;在HPA1-17中,杂合度最高的是HPA15,基因型HPA15a/15a、HPA15a/15b、HPA15b/15b频率分别是0.2750、0.5300、0.1950。结论分析探知了淄博地区血小板捐献者HLA-A,B和HPA1-17的分布特点。本研究将为建立山东省区域性血小板捐献者资料库提供帮助。
Objective To understand the genetic polymorphism of HLA-A,B alleles and HPA1-17 in platelet donors in Zibo area,and establish a database of platelet donors with HLA and HPA locus.Methods Polymerase chain reaction-sequencing based typing(PCR-SBT)and Polymerase chain reaction-sequence specific primer(PCR-SSP)methods were applied in genotyping for HLA-A,B and HPA1-17,respectively.Total of 200 blood samples were collected from unrelated platelet donors.The gene frequencies of HLA-A、B and HPA1-17 were calculated using direct counting method.Results The numbers of low-resolution alleles identified were 15 for HLA-A and 29 for HLA-B,respectively.The most frequent alleles at HLA-A locus were A^*02、A^*24、A^*11、A^*30、A^*33,with a gene frequency in 0.2600、0.1725、0.1375、0.1350、0.0700,respectively.For HLA-B locus,the common alleles were B^*13、B^*15、B^*40、B^*44、B^*51,with a gene frequency of 0.1600、0.1325、0.1300、0.0675、0.0625,respectively.Within HPA4a,7a-14a,16a and 17a systems,only HPA-aa genotype were found.Findings of HPA-15 showed the greatest heterozygosity with a genotype frequency of 0.2750、0.5300 and 0.1950 for HPA15a/15a,HPA15a/15b,and HPA15b/15b,respectively.Conclusion The distribution of HLA-A,B and HPA1-17 in platelet donors in Zibo area have been explored.This research can contribute to establish a regional bank of platelet donors in Shandong province.
作者
周博
田茂生
马晨
任晓朦
朱传福
ZHOU Bo;TIAN Maosheng;MA Chen(Zibo Blood Center,Zibo 255033)
出处
《临床输血与检验》
CAS
2020年第5期510-514,共5页
Journal of Clinical Transfusion and Laboratory Medicine
基金
山东省医药卫生科技发展基金项目(No.2013WS0169)
淄博市科学技术发展计划项目(No.2017kj010013)资助。
