摘要
目的:探讨一种酸枣仁提取物、茶氨酸、γ-氨基丁酸配方产品改善睡眠作用。方法:180只健康雄性Balb-c小鼠按体重随机分为三个大组,每个大组60只小鼠分别随机分为空白对照组和低、中、高3个剂量组,每组15只。酸枣仁提取物、茶叶茶氨酸和GABA配方产品按照低、中、高3个剂量(95、190、285 mg/kg·bw)通过直接睡眠、延长戊巴比妥钠睡眠时间、戊巴比妥钠阈下催眠剂量和巴比妥钠睡眠潜伏期实验,研究酸枣仁提取物、茶氨酸、γ-氨基丁酸复合配方对小鼠睡眠的影响。结果:酸枣仁提取物、茶氨酸与γ-氨基丁酸配方产品无直接催眠作用。与对照组相比,低、中、高剂量组小鼠戊巴比妥钠睡眠时间分别延长14.53%、17.12%、23.80%,且差异均具有统计学意义(P<0.05)。各剂量组小鼠30 min内小鼠阈下剂量实验均为阳性,小鼠睡眠发生率增加,差异具有统计学意义(P<0.05)。各剂量组小鼠巴比妥钠睡眠潜伏期时间分别缩短12.48%、24.19%、24.07%,差异有统计学意义(P<0.05)。结论:酸枣仁提取物、茶氨酸、γ氨基丁酸配方产品具有缩短小鼠睡眠潜伏期,延长小鼠睡眠时间,同时增加小鼠入睡发生率的作用,具有改善睡眠的功效。
Objective:To research the sleep improvement of Semen Ziziphi Spinosae,theanine and γ-aminobutyric acid formula products in mice.Methods:180 healthy male Balb-c mice were divided randomly into three groups.Each group was divided into four groups:One control group and three treatment group with equal size.Semen Ziziphi Spinosae,theanine and γ-aminobutyric acid were mixed in proportion and fed to mice at 95,190,285 mg/kg·bw respectively for 30 d by oral administration.Then direct sleep test,sleep extending test with sodium pentobarbital,hypnosis test under subthreshold dose of sodium pentobarbital and incubation period test with barbital sodium were conducted respectively.Results:The experimental samples had no direct hypnotic effect.The sleep time of mice injected with pentobarbital sodium in the low,medium and high dose group was longer by 14.53%,17.12%,23.80%compared with the model group(P<0.05).The rate of sleeping increased under the threshold hypnogenesis dosage of pentobarbital sodium in medium and high dose group(P<0.05).The sleeping delitescence induced by tobarbital sodium significant decreased by 12.48%,24.19%,24.07%compared with the model group(P<0.05).Conclusion:The formula consists of Semen Ziziphi Spinosae,theanine andγ-aminobutyric acid can shorten the sleep incubation period,prolong the sleep time and increase the sleeping rate of mice.According the results,the formula has the sleep improvement effect in mice.
作者
陈希民
王鑫
魏九龄
陈月晓
马姗婕
何梅
李东
CHEN Xi-min;WANG Xin;WEI Jiu-ling;CHEN Yue-xiao;Ma Shan-jie;HE Mei;LI Dong(Beijing Institute of Nutritional Sources,Beijing 100069,China)
出处
《食品工业科技》
CAS
北大核心
2020年第20期303-306,共4页
Science and Technology of Food Industry
关键词
酸枣仁
茶氨酸
Γ-氨基丁酸
改善睡眠
Semen Ziziphi Spinosae
theanine
γ-aminobutyric acid
sleep improvement
