摘要
目的:探讨龙须菜多糖(GPs)对D-半乳糖(D-gal)及氯化铝(AlCl3)诱导的大鼠神经毒性的保护效果。方法:采用腹腔注射D-gal及AlCl342 d的方法建立阿尔茨海默病(AD)大鼠模型。将大鼠分为对照组、AD组、AD+GPs(300 mg/kg,口服)组及AD+GPs(600 mg/kg,口服)组。采用Morris水迷宫(MWM)评估动物行为反应,进行海马氧化应激参数及组织病理学研究。结果:与AD组相比,口服GPs在获得性训练的第2、3及4 d显著地降低了平均逃避潜伏期(低剂量GPs组与AD组相比:相应地,P<0.05、0.05及0.01;高剂量GPs组与AD组相比:相应地,P<0.01、0.01及0.01)。在空间探索实验中,GPs组大鼠穿越平台次数极显著地高于AD组(P<0.01)。与AD组相比,低剂量及高剂量GPs均能很好地维持锥体神经元的有序排列。口服GPs可极显著地缓解海马超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性的降低(P<0.01)。此外,口服GPs还可极显著地抑制D-gal及AlCl3诱导的丙二醛(MDA)水平的升高(P<0.01)。结论:GPs可保护D-gal及AlCl3毒性所引起的大鼠认知损伤及海马组织形态学改变。GPs的神经保护效果可能通过其抗氧化及自由基清除作用来介导。
Objective:To explore the protective effects of Gracilaria lemaneiformis polysaccharides(GPs)on D-galactose(D-gal)and aluminumchloride(AlCl3)induced neurotoxicity in rats.Methods:Alzheimer’s disease(AD)rats was established by injecting both D-gal and AlCl3 into abdominal cavity for 42 d.Rats were divided into control group,AD group,AD+GPs(300 mg/kg,p.o.)group and AD+GPs(600 mg/kg,p.o.)group.Behavioral responses of animals were assessed in a Morris water maze(MWM).Oxidative stress parameters and histopathological studies were carried out in hippocampus.Results:Oral administration of GPs significantly decreased mean escape latency time as compared to the AD group on the second,third,fourth day of the acquisition trial(low-dose GPs group vs.the AD group:P<0.05,0.05 and 0.01,respectively;high-dose GPs group vs.the AD group:P<0.01,0.01 and 0.01,respectively).In the spatial exploration test,rats treated with GPs got higher numbers of platform crossings than that in the AD group(P<0.01).Compared with the AD group,both low-dose and highdose GPs treatments could well maintain the ordered arrangement of pyramidal neurons.The reduction of the hippocampus superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities were ameliorated by oral administration of GPs(all P<0.01).Furthermore,oral administration of GPs significantly prevented D-gal and AlCl3-induced rise in malondialdehyde(MDA)level(all P<0.01).Conclusion:GPs could protect cognitive impairments and morphological alterations caused by D-gal and AlCl3 toxicity in rats.The neuroprotective effect of GPs might be mediated through its antioxidant and free radical scavenging effects.
作者
陈茜
李嘉慈
吕应年
吴科锋
叶华
李立
CHEN Xi;LI Jia-ci;LV Ying-nian;WU Ke-feng;YE Hua;LI Li(The First Clinical Medical College,Guangdong Medical University,Zhanjiang 524523,China;Medical College of Shaoguan University,Shaoguan 512026,China;Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical University,Zhanjiang 524523,China;Marine Biomedical Research Institute,Guangdong Medical University,Zhanjiang 524523,China)
出处
《食品工业科技》
CAS
北大核心
2020年第20期290-294,302,共6页
Science and Technology of Food Industry
基金
广东省科技计划项目(2016B030309002)
湛江市科技计划项目(2017A06012)。
关键词
龙须菜
多糖
阿尔茨海默病
认知功能
氧化应激
Gracilaria lemaneiformis
polysaccharides
Alzheimer’s disease
cognitive function
oxidative stress
