摘要
目的初步探讨外周血的CD4^+CD25^+调节性T细胞(Tregs)在初治和复发自身免疫性溶血性贫血(AIHA)发病机制中的可能关系。方法选择2009年1月~2019年11月就诊于汕头大学医学院附属粤北人民医院的28例AIHA患者,通过流式细胞术分别检测患者在初发病时、治疗后达完全缓解时、复发时的外周血CD4^+T细胞和Tregs,并与28例年龄和性别相匹配的健康对照组结果对比分析。结果(1)AIHA初治组和复发组CD4^+T细胞比例分别为(26.42±11.38)%和(25.35±10.86)%,均较健康对照组(36.67±7.71)%低,差异有统计学意义(P<0.05)。(2)AIHA初治组Tregs比例(3.27±2.49)%,较完全缓解组(5.25±3.54)%和健康对照组(5.89±2.88)%均低,差异有统计学意义(P<0.05);复发组Tregs比例(3.19±2.12)%较完全缓解组(5.25±3.54)%和健康对照组(5.89±2.88)%均低,差异有统计学意义(P<0.05)。而AIHA初治组Tregs比例(3.27±2.49)%与复发组(3.19±2.12)%比较,差异无统计学意义(P>0.05);完全缓解组Tregs比例(5.25±3.54)%与健康对照组(5.89±2.88)%比较,差异无统计学意义(P>0.05)。结论AIHA初发病和复发时Tregs比例较完全缓解时比正常人低,而治疗达完全缓解时明显上升,与正常人无明显差异,提示Tregs与AIHA的细胞免疫紊乱可能有关。
Objective To research the possible relationship between CD4^+CD25^+regulatory T cells(Tregs)in peripheral blood and the pathogenesis of initially treated and relapsed autoimmune hemolytic anemia(AIHA).Methods From January 2009 to November 2019,28 patients with AIHA admitted to Yue Bei People’s Hospital Affiliated to Shantou University Medical College were collected.The CD4^+T cells and Tregs in peripheral blood of patients were detected by flow cytometry at the initial onset,complete remission after treatment and relapse,and the results were compared with 28 cases of the healthy control group with matching age and sex.Results(1)The ratios of CD4^+T cells in the AIHA initially treated group and the AIHA relapse group were(26.42±11.38)%and(25.35±10.86)%respectively,which were lower than(36.67±7.71)%in the healthy control group,and the differences were statistically significant(P<0.05).(2)The ratio of Tregs in AIHA initially treated group was(3.27±2.49)%,which was lower than(5.25±3.54)%in the complete remission group and(5.89±2.88)%in the healthy control group,and the differences were statistically significant(P<0.05).The ratio of Tregs in the relapse group was(3.19±2.12)%,which was lower than(5.25±3.54)%in the complete remission group and(5.89±2.88)%in the healthy control group,and the differences were statistically significant(P<0.05).However,there was no significant difference in the ratio of Tregs between AIHA initially treated group(3.27±2.49)%and the relapse group(3.19±2.12)%(P>0.05).The ratio of Tregs in the complete remission group was(5.25±3.54)%and(5.89±2.88)%in the healthy control group,and there was no statistically significant difference(P>0.05).Conclusion The ratio of Tregs is lower at the initial onset and relapse of AIHA than that at complete remission and normal people,but increases significantly at complete remission after treatment,with no statistically significant difference from normal people,suggesting that Tregs may be related to cellular immune disorder of AIHA.
作者
肖捷
杨丽萍
肖健
黄继贤
XIAO Jie;YANG Liping;XIAO Jian;HUANG Jixian(Medical Examination Center,Yuebei People's Hospital Affiliated to Shantou University Medical College,Guangdong,Shaoguan 512025,China;Department of Hematology,Yuebei People's Hospital Affiliated to Shantou University Medical College,Guangdong,Shaoguan 512025,China;Department of Medicine,Yuebei People's Hospital Affiliated to Shantou University Medical College,Guangdong,Shaoguan 512025,China)
出处
《中国医药科学》
2020年第16期199-202,共4页
China Medicine And Pharmacy
基金
广东省医学科研基金课题(A2009679)。
