摘要
目的探讨自身免疫性溶血性贫血(AIHA)/Evans综合征(AIHA同时或相继发生免疫性血小板减少性紫癜)患者外周血淋巴细胞共刺激分子CD80、CD86和CD4+CD25+调节性T细胞的表达水平,初步探讨共刺激分子CD80、CD86和CD4+CD25+调节性T细胞在AIHA/Evans综合征细胞免疫功能紊乱中的机制及其意义。方法应用流式细胞术检测24例次AIHA/Evans综合征患者治疗前后外周血淋巴细胞CD80、CD86和CD4+CD25+调节性T细胞比例的变化,并与正常对照组相比较。结果 AIHA/Evans综合征患者治疗前后与正常对照组比较CD80比例水平均无明显变化(P>0.05);发作时CD86表达水平高于对照组和治疗缓解组,差异具有统计学意义(P<0.05);CD4+CD25+调节性T细胞表达水平在AIHA/Evans综合征发作患者中表达明显下降,差异具有高度统计学意义(P<0.01)。结论淋巴细胞共刺激因子CD86和CD4+CD25+调节性T细胞异常表达可能参与AIHA/Evans综合征细胞免疫功能紊乱方面的发病机制。
Objective To explore the change of peripheral blood lymphocyte CD80,CD86,CD4 ＋ CD25 ＋ regulatory T cells and its mechanism in patients with autoimmune hemolytic anemia （AIHA）/Evans syndrome, remission patients and untreated patient. Methods The pro- portions of CD80,CD86 and CD4 ＋ CD25 ＋ regulatory T cells were determined in patients with AIHA/Evans syndrome, the remission patients after treatment and normal controls by flow cytometry. The proportions of these cells were compared among the three groups. Results There is no statis- tical difference between the patients with AIHA/Evans syndrome and normal controls in the proportions of the lymphocyte CD80（ P 〉0.05 ）. The proportions of the lymphocyte CD86 in patients is significantly higher than that of normal controls （ P 〈 0.05 ）. The proportions of CD4 ＋ CD25 ＋ regulatory T cells in patients is significantly lower than that of normal controls （ P 〈 0.01 ）. And the proportions of CD4 ＋ CD25 ＋ regulatory T cells in the remission patients after treatment is higher than that of untreated patients （ P 〈 0.05 ）. Conclusion There is abnormal about CD86 and CD4 ＋ CD25 ＋ regulatory T cells in patients with A1HA/Evans syndrome. CD86 and CD4 ＋ CD25 ＋ regulatory T cells may be involved in patbophysiology and mechanism of AIHA/Evans syndrome.
出处
《临床和实验医学杂志》
2013年第8期561-563,共3页
Journal of Clinical and Experimental Medicine
基金
2009年度广东省医学科研基金立项课题
项目编号:A2009679
