摘要
目的:采用高尿酸血症模型大鼠研究抑酸复合茶对高尿酸血症的防治作用,并探讨其降尿酸的作用机制。方法:大鼠适应性饲养1周后根据随机分组原则分为正常对照组、模型组、别嘌醇组及抑酸复合茶高、低剂量组,每组各12只。除正常对照组外,其余各组均采用腺嘌呤联合氧嗪酸钾连续灌胃3周以建立高尿酸血症模型,建模同时给予药物干预,观察各组大鼠给药前后血尿酸(UA)及给药后尿素氮(BUN)、肌酐(Scr)、胱抑素C(Cys C)、一氧化氮(NO)的变化。采用Western blot法检测抑酸复合茶对HEK293细胞URAT1和GLUT9表达的影响。结果:给药后,与模型组比较,抑酸复合茶高剂量组UA、BUN、Scr、Cys C均降低,NO升高,差异均有统计学意义(P <0.05);低剂量组UA、BUN降低,NO升高,差异亦有统计学意义(P <0.05);与别嘌醇组比较,抑酸复合茶高剂量组Cys C下降,NO升高,差异有统计学意义(P <0.05);抑酸复合茶还可使HEK293细胞中URAT1和GLUT9的表达量降低。结论:抑酸复合茶对高尿酸血症大鼠降尿酸作用明显,且对血管内皮细胞和肾功能具有一定的保护作用;其作用机制可能是通过下调肾脏URAT1和GLUT9蛋白表达而发挥。
Objective: To investigate the role of Yi-Suan-Cha in the prevention and treatment of hyperuricemia and its mechanism of action in reducing uric acid( UA) in a rat model of hyperuricemia. Methods: After one week of adaptive feeding,the rats were randomly divided into normal control group,model group,allopurinol group,and high-and low-dose Yi-Suan-Cha groups,with 12 rats in each group. All rats except those in the normal control group were given adenine combined with oteracil potassium by gavage for 3 consecutive weeks to establish a model of hyperuricemia,and drug intervention was given at the same time of modeling. Blood UA,blood urea nitrogen( BUN),serum creatinine( Scr),cystatin C( CysC),and nitric oxide( NO) were observed before and after administration. Western blot was used to evaluate the effect of Yi-Suan-Cha on the expression of URAT1 and GLUT9 in HEK293 cells. Results: Compared with the model group after administration,the high-dose Yi-Suan-Cha group had a significant reduction in UA,BUN,Scr,and CysC and a significant increase in NO( P < 0. 05),and the low-dose Yi-suan-Cha group had a significant reduction in UA and BUN and a significant increase in NO( P < 0. 05). Compared with the allopurinol group,the high-dose Yi-Suan-Cha group had a significant reduction in CysC and a significant increase in NO( P < 0. 05). Yi-Suan-Cha reduced the expression of URAT1 and GLUT9. Conclusion: Yi-Suan-Cha has a marked effect in reducing UA in rats with hyperuricemia and can exert a certain protective effect on vascular endothelial cells and renal function,possibly by downregulating the protein expression of URAT1 and GLUT9 in the kidney.
作者
陶慧
覃媛媛
蒙健军
林发全
TAO Hui;QIN Yuanyuan;MENG Jianjun;LIN Faquan(The First Ailiated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Guangxi Medical College,Nanning 530021,Cuangxi,China)
出处
《湖南中医杂志》
2020年第8期160-162,176,共4页
Hunan Journal of Traditional Chinese Medicine
基金
广西壮族自治区自然科学基金项目(2017GXNSFAA198150)。
